the chromatin remodelling complex b-wich changes the chromatin structure and recruits histone acetyl-transferases to active rrna genes染色质重塑复合物b-wich改变染色质结构和招聘组蛋白acetyl-transferases主动核糖体rna基因.pdfVIP

The Chromatin Remodelling Complex B-WICH Changes the Chromatin Structure and Recruits Histone Acetyl-Transferases to Active rRNA Genes 1 ¨ 1 1 2 ´ 1 Anna Vintermist , Stefanie Bohm , Fatemeh Sadeghifar , Emilie Louvet , Anethe Mansen , Pergiorgio 2 ¨ 1 Percipalle , Ann-Kristin Ostlund Farrants * 1 Department of Cell Biology, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden, 2 Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden Abstract The chromatin remodelling complex B-WICH, which comprises the William syndrome transcription factor (WSTF), SNF2h, and nuclear myosin 1 (NM1), is involved in regulating rDNA transcription, and SiRNA silencing of WSTF leads to a reduced level of 45S pre-rRNA. The mechanism behind the action of B-WICH is unclear. Here, we show that the B-WICH complex affects the chromatin structure and that silencing of the WSTF protein results in a compaction of the chromatin structure over a 200 basepair region at the rRNA promoter. WSTF knock down does not show an effect on the binding of the rRNA- specific enhancer and chromatin protein UBF, which contributes to the chromatin structure at active genes. Instead, WSTF knock down results in a reduced level of acetylated H3-Ac, in particular H3K9-Ac, at the promoter and along the gene. The association of the histone acetyl-transferases PCAF, p300 and GCN5 with the promoter is reduced in WSTF knock down cells, whereas the association of the histone acetyl-transferase MOF is retained. A low level of H3-Ac was also found in growing cells, but here histone acetyl-transferases were present at the rDNA promoter. We propose that the B-WICH complex remodels the ch