the hschsp70 co-chaperone network controls antigen aggregation and presentation during maturation of professional antigen presenting cells期间的hschsp70 co-chaperone网络控制抗原聚合和呈现成熟专业的抗原呈递细胞.pdfVIP

The Hsc/Hsp70 Co-Chaperone Network Controls Antigen Aggregation and Presentation during Maturation of Professional Antigen Presenting Cells 1 1 2 ¨ 3 ¨ ¨ 1 Nadja Kettern , Christian Rogon , Andreas Limmer , Hansjorg Schild , Jorg Hohfeld * 1 Institute for Cell Biology, Rheinische Friedrich-Wilhelms-University Bonn, Bonn, Germany, 2 Institute for Molecular Medicine and Experimental Immunology, University Hospital Bonn, Bonn, Germany, 3 Institute for Immunology, University of Mainz, Mainz, Germany Abstract The maturation of mouse macrophages and dendritic cells involves the transient deposition of ubiquitylated proteins in the form of dendritic cell aggresome-like induced structures (DALIS). Transient DALIS formation was used here as a paradigm to study how mammalian cells influence the formation and disassembly of protein aggregates through alterations of their proteostasis machinery. Co-chaperones that modulate the interplay of Hsc70 and Hsp70 with the ubiquitin-proteasome system (UPS) and the autophagosome-lysosome pathway emerged as key regulators of this process. The chaperone- associated ubiquitin ligase CHIP and the ubiquitin-domain protein BAG-1 are essential for DALIS formation in mouse macrophages and bone-marrow derived dendritic cells (BMDCs). CHIP also cooperates with BAG-3 and the autophagic ubiquitin adaptor p62 in the clearance of DALIS through chaperone-assisted selective autophagy (CASA). On the other hand, the co-chaperone HspBP1 inhibits the activity of CHIP and thereby attenuates antigen sequestration. Through a modulation of DALIS formation CHIP, BAG-1 and HspBP1 alter MHC class I mediated antigen presentation in mouse BMDCs. Our data show that t