unusual interferon gamma measurements with quantiferon-tb gold and quantiferon-tb gold in-tube tests不寻常的干扰素γ测量与quantiferon-tb黄金和quantiferon-tb黄金管测试.pdfVIP

Unusual Interferon Gamma Measurements with QuantiFERON-TB Gold and QuantiFERON-TB Gold In- Tube Tests 1 1 2 1 1 Richard D. Powell III , William C. Whitworth , John Bernardo , Patrick K. Moonan , Gerald H. Mazurek * 1 Division of Tuberculosis Elimination, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 The Pulmonary Center, Boston University School of Medicine, Boston, Massachusetts, United States of America Abstract Introduction: Interferon gamma (IFN-c) release assays, such as QuantiFERONH-TB Gold test (QFT-G) and QuantiFERONH-TB Gold In-Tube test (QFT-GIT) are designed to detect M. tuberculosis (Mtb) infection. Recognition of unusual IFN-c measurements may help indicate inaccurate results. Methods: We examined QFT-G and QFT-GIT results from subjects who had two or more tests completed. We classified unusual IFN-c measurements as: 1) High Nil Concentration (HNC) when IFN-c concentration in plasma from unstimulated blood exceeded 0.7 IU/mL; 2) Low Mitogen Response (LMR) when Mitogen Response was ,0.5 IU/mL; 3) Very Low Mitogen Response (VLMR) when Mitogen Response was #20.5 IU/mL; and 4) Very Low Antigen Response (VLAR) when the response to a Mtb antigen was #20.35 IU/mL and #20.5 times the IFN-c concentration in plasma from unstimulated blood. Results: Among 5,309 results from 1,728 subjects, HNC occurred in 234 (4.4%) tests for 162 subjects, LMR in 108 (2.0%) tests for 85 subjects, VLMR in 22 (0.4%) tests for 21 subjects, and VLAR in 41 (0.8%) tests for 39 subjects. QFT-GIT had fewer HNC, VLMR, and VLAR (p = 0.042, 0.004, and 0.067 respectively); QFT-G had fewer LMR (p = 0.005). Twenty-f