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using complementary acoustic and optical techniques for quantitative monitoring of biomolecular adsorption at interfaces使用互补的声学和光学技术的定量监测生物分子吸附在界面
Biosensors 2012, 2, 341-376; doi:10.3390/bios2040341
OPEN ACCESS
biosensors
ISSN 2079-6374
/journal/biosensors/
Review
Using Complementary Acoustic and Optical Techniques for
Quantitative Monitoring of Biomolecular Adsorption at Interfaces
Rupert Konradi 1, Marcus Textor 2 and Erik Reimhult 3,*
1 BASF SE, Advanced Materials and Systems Research, D-67056, Ludwigshafen, Germany;
E-Mail: rupert.konradi@
2 Laboratory for Surface Science and Technology, Department of Materials,
Swiss Federal Institute of Technology (ETH) Zurich, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich,
Switzerland; E-Mail: marcus.textor@mat.ethz.ch
3 Laboratory for Biologically inspired materials, Department of Nanobiotechnology, University of
Natural Resources and Life Sciences Vienna, A-1190 Vienna, Austria
* Author to whom correspondence should be addressed; E-Mail: erik.reimhult@boku.ac.at;
Tel.: +43-1-47654-2230; Fax: +43-1-47891-12.
Received: 31 July 2012; in revised form: 27 August 2012 / Accepted: 3 September 2012 /
Published: 26 September 2012
Abstract: The great wealth of different surface sensitive techniques used in biosensing,
most of which claim to measure adsorbed mass, can at first glance look unnecessary.
However, with each technique relying on a different transducer principle there is
something to be gained from a comparison. In this tutorial review, different optical and
acoustic evanescent techniques are used to illustrate how an understand
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