very high throughput electrical cell lysis and extraction of intracellular compounds using 3d carbon electrodes in lab-on-a-chip devices非常高的吞吐量电气细胞溶菌作用和细胞内化合物的提取使用3 d碳电极芯片实验室设备.pdfVIP

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very high throughput electrical cell lysis and extraction of intracellular compounds using 3d carbon electrodes in lab-on-a-chip devices非常高的吞吐量电气细胞溶菌作用和细胞内化合物的提取使用3 d碳电极芯片实验室设备.pdf

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very high throughput electrical cell lysis and extraction of intracellular compounds using 3d carbon electrodes in lab-on-a-chip devices非常高的吞吐量电气细胞溶菌作用和细胞内化合物的提取使用3 d碳电极芯片实验室设备

Micromachines 2012, 3, 574-581; doi:10.3390/mi3030574 OPEN ACCESS micromachines ISSN 2072–666X /journal/micromachines Communication Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices Guillaume Mernier 1,†, Rodrigo Martinez-Duarte 1,†,*, Rajwinder Lehal 2, Freddy Radtke 2 1 and Philippe Renaud 1 Microsystems Laboratory LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland; E-Mails: guillaume.mernier@epfl.ch (G.M.); philippe.renaud@epfl.ch (P.R.) 2 Swiss Institute for Experimental Cancer Research, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, Lausanne, Vaud 1015, Switzerland; E-Mails: rajwinder.lehal@epfl.ch (R.L.); freddy.radtke@epfl.ch (F.R.) † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: drmartnz@; Tel.: +41-21-69-36582; Fax: +41-21-69-35950. Received: 25 June 2012; in revised form: 30 July 2012 / Accepted: 3 August 2012 / Published: 16 August 2012 Abstract: Here we present an electrical lysis throughput of 600 microliters per minute at 8 high cell density (10 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as