design and implementation of degenerate microsatellite primers for the mammalian clade简并微卫星引物的设计和实现哺乳动物的进化枝.pdfVIP

Design and Implementation of Degenerate Microsatellite Primers for the Mammalian Clade Emmanuel Buschiazzo*¤a, Josephine S. Beck, Neil J. Gemmell¤b School of Biological Sciences, University of Canterbury, Christchurch, New Zealand Abstract Microsatellites are popular genetic markers in molecular ecology, genetic mapping and forensics. Unfortunately, despite recent advances, the isolation of de novo polymorphic microsatellite loci often requires expensive and intensive groundwork. Primers developed for a focal species are