discovery and potential of snp markers in characterization of tunisian olive germplasm发现和潜在的snp标记在突尼斯油橄榄种质特性.pdfVIP

Diversity 2010, 2, 17-27; doi:10.3390/d2010017 OPEN ACCESS diversity ISSN 2071-1050 /journal/diversity Article Discovery and Potential of SNP Markers in Characterization of Tunisian Olive Germplasm 1,2 1 1 2, Imen Rekik Hakim , Naziha Grati Kammoun , Emna Makhloufi and Ahmed Rebaï * 1 Institut de lOlivier, P.O. Box 1087, 3018 Sfax, Tunisia; E-Mails: imenbmc@yahoo.fr (I.R.H.); kamoun_naziha@yahoo.fr (N.G.K.); bioinfomatics@cbs.rnrt.tn (E.M.) 2 Centre de Biotechnologie de Sfax, P.O. Box 1177, 3038 Sfax, Tunisia * Author to whom correspondence should be addressed; E-Mail: ahmed.rebai@cbs.rnrt.tn; Tel.: +21-698817294; Fax: +21-674875818. Received: 10 November 2009 / Accepted: 22 December 2009 / Published: 30 December 2009 Abstract: Single Nucelotide Polymorphisms (SNPs) have become the most widely used markers in many current genetic applications. Here we report the discovery of nine new SNPs in olives by direct partial sequencing of two genes (OEX and OEW) in sixteen Tunisian cultivars. The SNP markers were then used to genotype 24 olive cultivars and assess the level of genetic diversity. Power of discrimination of SNP markers was then compared to that of microsatellites (SSRs). A combination of SSR and SNP markers was finally proposed that can be used for cultivars identification in juvenile step or