dna repair in human pluripotent stem cells is distinct from that in non-pluripotent human cellsdna修复人类多能干细胞是有别于non-pluripotent人类细胞.pdfVIP

DNA Repair in Human Pluripotent Stem Cells Is Distinct from That in Non-Pluripotent Human Cells 1 2. 1. 1 1 Li Z. Luo , Sailesh Gopalakrishna-Pillai , Stephanie L. Nay , Sang-Won Park , Steven E. Bates , 3 2 1 Xianmin Zeng , Linda E. Iverson , Timothy R. O’Connor * 1 Department of Cancer Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California, United States of America, 2 Department of Stem Cell Biology, Beckman Research Institute, City of Hope National Medical Center, Duarte, California, United States of America, 3 North Bay CIRM Shared Research Laboratory for Stem Cells and Aging, Buck Institute for Age Research, Novato, California, United States of America Abstract The potential for human disease treatment using human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells (iPSCs), also carries the risk of added genomic instability. Genomic instability is most often linked to DNA repair deficiencies, which indicates that screening/characterization of possible repair deficiencies in pluripotent human stem cells should be a necessary step prior to their clinical and research use. In this study, a comparison of DNA repair pathways in pluripotent cells, as compared to those in non-pluripotent cells, demonstrated that DNA repair capacities of pluripotent cell lines were more heterogeneous than those of differentiated lines examined and were generally greater. Although pluripotent cells had high DNA repair capacities for nucleotide excision repair, we show that ultraviolet radiation at low fluxes induced an apoptotic response in these cells, while differentiated