蛋白质含量测定法.doc（蛋白质含量测定法.doc）.docVIP

蛋白质含量测定法.doc（蛋白质含量测定法.doc） Appendix protein assay First Kjeldahl method 1 precipitation with tungstic acid The total protein content of the protein in the filtrate of the sample removed by the precipitation of tungstate and the content of non protein nitrogen in the filtrate were calculated. Preparation of test sample solution (1) determination of total nitrogen, preparation of solution, precision sample, test sample 1ml, accurate dilution to Sodium Chloride Physiological Solution with nitrogen content of about 1mg per 1ml. (2) the preparation of non protein nitrogen determination precision, take the test product 2ml, add water 14ml, 10% sodium tungstate solution 2ml, sulfuric acid solution (1.86 to 100) 2ml, shake it well, stand aside for 30 minutes to filter, take the filtrate determination. The total nitrogen content in the tested product was determined by the precision method, the total nitrogen determination solution 1ml, the Kjeldahl flask, and the nitrogen determination method (Appendix VI, A). At the same time blank control. The non protein nitrogen determination solution 5ml was used to determine the non protein nitrogen content of the tested products in the Kjeldahl flask and the nitrogen determination method (Appendix VI, A). Press down calculation CTN (mg/ml) = (VX1-V0) * c * 14.01 * n * 2 _________________________ V1 CNPN (mg/ml) = (VX2-V0) * c * 14.01 * n * 2 _________________________ V2 CPN (mg/ml) =CTN-CNPN Protein content of the test sample (%, g/ml) =CPN * 6.25 * 100 _______________ One thousand CTN was the test sample in the formula, and the total nitrogen content of solution was mg/ml; CNPN was the test solution, non protein nitrogen content, mg/ml; VX1 as a test product, total nitrogen was measured, the solution consumed, and the volume of acid titration liquid, ml; VX2 as a test sample, non protein nitrogen determination, solution consumption, acid titration volume, ml; V0 is the blank test consuming the volume of acid titration liquid, ml; C i