褪黑素对UUO所致肾脏氧化损伤及血液流变学变化影响.doc

褪黑素对UUO所致肾脏氧化损伤及血液流变学变化影响 　 作者：刘昌明, 华春秀, 李著华 【摘要】 　　目的 探讨褪黑素对单侧输尿管梗阻(Unilateral ureteral obstruction，UUO)所致肾脏氧化损伤及机体血液流变学的影响。 方法 成年健康雌性SD大鼠30只，随机分为三组：假手术组（Sham）、模型组（UUO ）和褪黑素组（Mel）各10只。UUO组和Mel组行左侧输尿管结扎， Sham组不结扎输尿管。Mel组于术后腹腔注射Mel（10 mg/kg ·d ），UUO组和Sham组给予等量含有5%乙醇的生理盐水溶液，连续14d后，取左肾皮质检测丙二醛(alondialdehvde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)活性；腹主动脉取血检测机体血液流变学变化。结果 UUO组与Sham组比较，肾组织中MDA含量增加，SOD活性降低，全血粘度、血浆粘度和全血还原粘度升高，红细胞压积、红细胞计数和红细胞聚集指数降低； Mel组与UUO组比较，肾脏肾组织中MDA含量降低，SOD活性升高，红细胞计数和红细胞压积增加，全血粘度和全血还原粘度降低。结论 Mel可以减轻UUO所致肾脏氧化应激损伤和改善UUO所致机体血液流变学变化，说明mel对梗阻性肾损伤具有明显的保护作用。 【关键词】 褪黑素 ; 单侧输尿管梗阻 ; 氧化应激 ; 血液流变学 　　Abstract Objective To study the effect of melatonin on renal oxidation damage and on the change of body hemorrheology induced by unilateral ureteral obstruction (UUO). Methods 30 female adult Sprague-Dawley rats were randomly divided into 3 groups: sham group, UUO group and melatonin group, 10 in each; left ureteral ligation was performed on rats in UUO group and melatonin group but not on rats in sham group; intraperitoneal injuection of melatonin (10mg/kg·d) was made to rats in melatonin after operation continuously for 14 days while the same volume of alcohol physiological saline was injected to rats in UUO group and sham group; the content of malordialdehyde and the activity of superoxide dismutase in left renal cortex were detected; blood sample was taken from abdominal aorta to detect the change of body hemorrheology. Results The content of MDA in nephridial tissue increased in UUO group while the activity of T-SOD decreased; the whole blood viscosity, plasma viscosity and the whole blood reduced viscosity increased while the hematocrit, red blood cell count and erythrocyte aggregation index decreased; in melatonin group, the content of MDA in nephridial tissue decreased, the whole blood viscosity and the whole blood reduced viscosity decreased as well while the activity of T-SOD, the red blood cell count and hematocrit increased. Conclusions Melatonin can