豚鼠海马神经干细胞及两种不同载体材料生物相容性.docVIP

豚鼠海马神经干细胞及两种不同载体材料生物相容性 　 作者：郭宝凤，董明敏，郭漳生 【关键词】 神经干细胞 Biocompatibility between two different biomaterials and cultured hippocampusderived neural stem cells in vitro 　　【Abstract】 AIM: To study the biocompatibility between two different biomaterials (collagen protein sponge and glutin sponge) and cultured hippocampusderived neural stem cells in vitro and to identify whether the materials could be used as scaffold biomaterials in peripheral neural tissue engineering. METHODS: Hippocampusderived neural stem cells of guinea pigs were cultured and respectively combined with each of the two biomaterials in vitro. The number of cells was counted and the histological changes were observed with HE staining and scanning electron microscope after 7 d and the adhesion rates were detected. RESULTS: Hippocampusderived neural stem cells attached to both biomaterials and normally grew. The adhesion rates were 31.17% and 14.87% respectively. The total numbers of the cells in the experimental and control groups were similar and there was no statistical difference. CONCLUSION: Both biomaterials have a good biocompatibility with hippocampusderived neural stem cells of guinea pigs and can be used safely as scaffold materials in peripheral neural tissue engineering. 　　【Keywords】 neuronal stem cells; tissue engineering; biomaterials; biocompatibility; guinea pig 　　【摘要】 目的：评价豚鼠海马神经干细胞与2种不同载体材料（胶原蛋白海绵和明胶海绵）的生物相容性,探讨它们作为周围神经组织工程载体材料的可行性. 方法：体外培养新生豚鼠海马神经干细胞，传至第3代，将密度为1×1010/L细胞分别与2种载体材料联合体外培养,1 wk后取材，进行细胞计数，倒置相差显微镜及扫描电镜观察,并测定2种材料与细胞的吸附率. 结果：神经干细胞可以在胶原蛋白海绵和明胶海绵上生长,逐渐黏附，细胞吸附率分别为37.17%和14.87%,统计学分析有显著差异；各实验组与对照组之间细胞总数统计学分析无显著差异. 结论：胶原蛋白及明胶2种材料尤其是胶原蛋白,具有良好的神经干细胞相容性,可以作为周围神经组织工程的支架材料应用于临床. 　　【关键词】 神经干细胞；组织工程；生物材料；生物相容性；豚鼠 　　0引言 　　在应用组织工程修复周围神经的研究中，理想的载体应与细胞外基质类似，与活体细胞有良好的生物相容性［1-2］. 胶原蛋白是细胞外基质的组成部分，它具有细胞外基质的共性，是细胞赖以生存和维持细胞功能及生物学特征的基础；明胶由胶原蛋白水解而得. 神经干细胞（nerve stem cells,NSCs）由于具有来源广泛、取材容易和多向分化潜能等特点,是一种理想的种子细胞［3-4］. 我们选择豚鼠海马NSCs与胶原、明胶2种载体材料进行体外复合培养，检测其组织相容性,以探讨它们作为周围神经组织工程载体材料的可行性. 　　1材料和方法 　　1.1材料