赤雹果不同提取物镇痛活性比较探究.docVIP

赤雹果不同提取物镇痛活性比较探究 　 作者：佟继铭 李兰芳 宋利萍 刘玉玲 陈光晖 毕业论文 【关键词】 赤雹果；,,提取物,；,,镇痛活性 论文代写 　　摘要：目的评价赤雹果不同提取物的镇痛活性，筛选镇痛活性强的提取物，为进一步提取和分离其镇痛活性部位或成分提供依据。 方法 采用小鼠扭体法、小鼠热板法对赤雹果石油醚提取物（F1）、醋酸乙酯提取物（F2）、正丁醇提取物（F3）、乙醇提取物（F4）和水提取物（F5）的镇痛活性进行了比较 研究 。结果所用的5种赤雹果提取物对2种疼痛模型均有一定的镇痛作用，对小鼠扭体反应的抑制率依次为： F553.6％，F339.4%，F436.3% ，F234.6%，F119.0%；在小鼠热板实验，痛阈提高率依次为：F554.6％，F4 42.9% ，F315.6%，F213.0%， F19.1%。结论 赤雹果5种提取物均有不同程度的镇痛活性，其中F5镇痛活性最强； F4和F3也有一定的镇痛活性，F2和F1的镇痛活性较弱，结果提示赤雹果镇痛活性物质主要存在于F5，其次是F4和 F3。 　　关键词：赤雹果； 提取物 ； 镇痛活性 毕业论文 　　 The Comparison of Analgesic Activity of Different Extracts of the Fruits of Thladiantha dubia Bunge 毕业论文 　　Abstract：ObjectiveTo evaluate the analgesic activity of the 5 extracts of the fruits of Thladiantha dubia Bunge and find out the strongest one from 5 extracts, to provide the foundation for further extracting and separating analgesic activity fraction or components.MethodsThe analgesic activity of 5 extracts extracted by petroleumetter (F1) ,ethyl acetate (F2), Nbutanol (F3),ethanol(F4) and water(F5) respectively were evaluated by the classical writhing test , hot plate test (55℃) in mice. ResultsIn the 2 pain models, the 5 extracts all have different analgesic activity. In the writhing test, inhibited rate of the number of writhing was F553.6％，F339.4%，F436.3%， F234.6%，F119.0%,respectively.In the hot plate test, pain threshold value was F554.6％，F442.9%，F315.6%， F213.0%， F19.1% respectively.ConclusionThe 5 extracts of the fruits of Thladiantha dubia Bunge all have different analgesic activity and analgesic activity of F5 is the strongest; F4 and F3 also have certain analgesic activity; analgesic activity of F2 and F1 is less strong. The results show that analgesic activity substance mainly consists in F5, and then in F4 and F3. 论文代写 　　Key words： Fruit of Thladiantha dubia Bunge ; Extract ; Analgesic activity 　　赤雹果The fruit of Thladiantha dubi Bunge, FTDB是葫芦科植物赤雹Thladiantha dubia，TDB的干燥成熟果实［1］，多年生蔓性草本，分布于华北东北等区，是满族人长期习用药材。具有降逆、理湿、化淤等功能，主治咳血胸痛、腰腿痛、痛经、软组织扭伤等多种疼痛