运动病大鼠血浆降钙素基因相关肽含量变化.docVIP

运动病大鼠血浆降钙素基因相关肽含量变化 　 作者：王小成，冯立宁，张作明 【关键词】 降钙素基因相关肽；晕动病；大鼠 　　Effect of rotatory stimulation on plasma level of calcitonin generelated peptide in rats 　　【Abstract】 AIM: To explore the relation between calcitonin generelated peptide (CGRP) and motion sickness（MS） in rats. METHODS: A group of 30 rats were subdivided into 4 experimental groups and a control group with a random number table. The rats of experimental groups were treated with rotatory stimulation for 30 min. Then the plasma levels of CGRP in 4 experimental groups were measured respectively 20 min, 6 h, 1 and 3 d after the stimulation.According to the changes of rats intake of saccharin solution before and 24 h after rotatory stimulation for 15 min. Another group of 20 rats were subdivided into a group sensitive to MS and a group insensitive to MS, then the plasma levels of CGRP in the 20 rats were measured 20 min after rotatory stimulation for 15 min. RESULTS: There was no significant difference in the plasma levels of CGRP at different time points after rotatory stimulation in the first group （F=1.105, P=0.893）; there was no significant difference in the plasma levels of CGRP between the group sensitive to MS and the group insensitive to MS （P=0.704）. CONCLUSION: The CGRP in blood plasma doesnt play an role in the pathogenesis of MS. 　　【Keywords】 calcitonin generelated peptide; motion sickness; rats 　　【摘要】目的： 探讨降钙素基因相关肽（CGRP）在运动病发病过程中的作用. 方法： 第1组30只大鼠按随机数字法分为4个实验组和1个对照组，实验组动物给予30 min旋转运动刺激，然后各自测4个实验组动物旋转运动刺激后20 min, 6 h, 1 d, 3 d以及对照组动物血浆CGRP含量；第2组20只大鼠先给予15 min的旋转运动刺激，根据刺激前后24 h糖精水饮用量的变化分为运动病敏感组和不敏感组，然后测两组动物旋转运动刺激15 min后血浆CGRP含量. 结果： 第1组动物，实验组动物旋转运动刺激30 min后各时间点大鼠血浆CGRP含量和对照组差异没有显著性意义（F=1.105, P=0.893）；第2组动物，运动病敏感组与运动病不敏感组相比，旋转运动刺激15 min后血浆CGRP含量差异无显著性意义（P=0.704）. 结论： 血浆CGRP在大鼠运动病发病机制中没有发挥作用；大脑中枢和前庭终器中CGRP是否参与了运动病的发病尚需深入研究. 　　【关键词】 降钙素基因相关肽；晕动病；大鼠 　　0引言 　　运动病一直是妨碍人类健康、降低航空航海作业能力和太空探索能力的一大难题，其发生率较高. 虽经国内外长期研究，但运动病的确切发病机制至今仍未阐明，使得其治疗和预防等研究难以深入［1-2］. 降钙素基因相关肽（calcitonin generelated peptide, CGRP）是近年来在人和哺乳动物