雷诺昔酚对去势后大鼠骨密度、血生化指标影响.docVIP

雷诺昔酚对去势后大鼠骨密度、血生化指标影响 　【关键词】 ,卵巢切除术 Influence of SERMraloxifene on bone mineral density and blood biochemical parameters in ovariectomized rats 　　【Abstract】 AIM: To study the influence of raloxifene (RAL), a selective estrogen receptor modulator (SERM), on bone mineral density（BMD） loss in cancellated bone and compact bone of ovariectomized rats. METHODS: Twentyfour female SD rats were divided into 3 groups (8 rats per group): sham operation group, ovariectomized（OVX） group and therapeutic（OVX+RAL） group. Bilateral ovariectomies were performed in OVX group and OVX+RAL group. The latter was treated with raloxifene 7 d after operation. Sixteen weeks after operation, all rats were sacrificed. BMD of L3L5 vertebrae, proximal femoral segment and femoral shaft were measured by dual energy Xray absorptiometry (DEXA). L2 vertebrae were histomorphometrically analyzed and serum biochemical markers were measured before and after drug administration. RESULTS: Serum total calcium ［（3.02±0.38） mmol/L vs（2.24±0.30） mmol/L］ and total alkaline phosphatase ［（2.336±0.032） μkat/L vs （1.832±0.028 ）μkat/L］ significantly increased in OVX group compared with those in sham group (Plt;0.01, Plt;0.05 respectively). The above parameters decreased in RAL group compared with those the ovariectomized group ［（2.38±0.39） mmol/L vs（3.02±0.38）mmol/L,（1.952±0.023） μkat/L vs （2.336±0.032） μkat/L］ (Plt;0.05). Sixteen weeks after ovariectomy, BMD of L3L5 vertebrae, proximal femoral segment ［（0.181±0.021） g/cm2 vs （0.287±0.025 ）g/cm2］ and femoral shaft ［（0.157±0.034） g/cm2 vs （0.260±0.039） g/cm2］ decreased significantly (Plt;0.01) and the trabecular became thin and irregular compared with those in sham group. After treatment of RAL, the above parameters restored (Plt;0.05) but not to the level of sham group (Pgt;0.05). CONCLUSION: Ovariectomy induces a significant decrease of cancellated bone mass and compact bone mass. RAL is an effective medicine to inhibit bone loss, increase the BMD of lumbar spine,