黄根醇提物对四氯化碳所致大鼠肝纤维化保护作用.docVIP

黄根醇提物对四氯化碳所致大鼠肝纤维化保护作用 　作者：邓家刚，周程艳，郑作文 【摘要】 　　目的研究黄根醇提物对四氯化碳所致大鼠肝纤维化的保护作用。方法大鼠首次于背部皮下注射纯CCl4 5 ml/kg，以后每周2次注射40%(V/V) CCl4花生油3 ml/kg，连续注射8周。8周后取血，计算大鼠的肝脾指数，检测大鼠血清中ALT，AST的活性及其总蛋白（TP）、白蛋白(Alb)的含量，计算白蛋白与球蛋白的比值；检测血清中透明质酸(HA)、层黏连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ) 的含量；检测肝脏组织中超氧化物歧化酶(SOD)活性、羟脯氨酸（Hyp）含量、丙二醛（MDA）和谷胱甘肽过氧化物酶(GSH-Px)水平。结果黄根醇提物能明显降低CCl4所致的大鼠肝纤维化肝脾指数的升高(Plt;0.01)和血清中ALT，AST活性的升高(Plt;0.01)，降低血清中HA，LN ，PCⅢ，CIV，GLB的含量(Plt;0.01)，升高血清中的TP，ALB含量和A/G的比值(Plt;0.01)；降低肝组织中MDA 的水平和Hyp 的含量(Plt;0.01)，亦能升高肝组织中SOD的活性和GSH-Px的水平(Plt;0.01)。结论黄根醇提物具有显著的抗肝纤维化的作用。 【关键词】 黄根 四氯化碳 肝纤维化 　　Abstract：ObjectiveTo study the protective effects of the alcohol extract from Prismatomeris tetrandra against liver fibrosis in mice induced by carbon tetrachlotide (CCl4). MethodsMouse model with liver fibrosis was induced by the multiple subcutaneous injections of 40% CCl4, 3 ml/kg, twice a week for 8weeks.The indexes of the liver and spleen in mice were calculated.ALT and AST activities and contents of TP，Alb in serum were examined,meanwhile the ratio of the ALB and GLB were calculated.The four indexes of liver- fibrosis(HA，LN，PCⅢ，CⅣ) were determined in liver tissue. SOD activities and MDA，GSH levels in liver tissue were determined. Results The alcohol extract from Prismatomeris tetrandra could obviously inhibit the increase of liver and spleen indexes(Plt;0.01)in liver fibrosis mice, and the increase of ALT and AST in serum (Plt; 0.01) , elevate the contents of TP，ALB ，A/G in serum(Plt;0.01),suppress the content of HA，LN ，PCⅢ，CIV ，GLB in liver tissue(Plt;0.01) , decrease the MDA levels in liver tissue(Plt;0.01), and improve SOD activity and GSH level in liver tissue(Plt;0.01) ConclusionThe alcohol extract from Prismatomeris tetrandra has remarkable protective effects on liver fibrosis in mice. 　　Key words:Alcohol extract from Prismatomeris tetrandra; Carbon tetrachlotide; Liver fibrosis 　　肝纤维化是慢性肝病重要的病理特征，也是进一步向肝硬化发展的主要中间环节［1］。目前研究认为肝纤维化是一个动态过程，尚属于可逆性病变，其转归与肝损伤因素是否持续存在有关，如果得到及时彻底治疗，肝损伤停止发展，则肝纤维化多可逆转；病情如不断