Cathepsin B、uPA及uPAR基因在肝细胞肝癌中表达、相互关系及作.doc

Cathepsin B、uPA及uPAR基因在肝细胞肝癌中表达、相互关系及作 　【摘要】 目的: 探讨肝细胞肝癌（HCC）组织中组织蛋白酶B(CB)、尿激酶型纤溶酶原激活物(uPA)及其受体（uPAR）基因的表达、相互关系及作用，并分析其机制。方法: 应用RT-PCR方法测定71例肝组织标本，包括对照组（C）12例，HCC组34例和癌旁组织（PTT）组25例的CB mRNA、uPA mRNA、uPAR mRNA表达，分析其间的相关性。结果: HCC组CB mRNA、uPA mRNA、uPAR mRNA表达均明显高于C组和PTT组，差异有显著性（P lt;0.01），PTT组与C组间差异均无显著性（P gt;0.05）。CB mRNA与uPA mRNA（r＝0.805），CB mRNA与uPAR mRNA（r＝0.786），uPA mRNA与uPAR mRNA（r＝0.874）之间均存在显著的正相关关系（均P lt;0.01）。结论: 肝癌组织CB mRNA、uPA mRNA、uPAR mRNA均呈现高表达，它们之间可能相互诱导，协同促使肝癌的侵袭和转移。 【关键词】 癌 肝细胞 组织蛋白酶B 尿纤溶酶原激活物 尿纤溶酶原激活物受体 Expression，relationship and effect of gene of cathepsin B，urokinase-type plasminogen activator（uPA）and its receptor（uPAR）in hepatocellular carcinoma XU Chang-long*，ZHENG Jun-jie，XUE Zhan-xiong，HUANG Zhi-ming．*Department of Digestive Medicine，the Second Affilated Hospital of Wenzhou Medical College，Wenzhou，325027 Abstract: Objective: To determine the expression, relationship and function of gene of cathepsin B (CB)，urokinase-type plasminogen activator（uPA）and its receptor（uPAR）in hepatocellular carcinoma，and to explore their effects and mechanism. Methods: The expression of CB mRNA，uPA mRNA and uPAR mRNA in 71 specimens of human hepatic tissue，including control (C) 12，hepatocellular carcinoma (HCC) 34 and paratumor tissue of hepatocellular carcinoma (PTT) 25 were detected with reverse transcription-polymerase chain raction (RT-PCR). The expression of relationship among CB mRNA,uPA mRNA and uPAR mRNA were researched. Results: The CB mRNA,uPA mRNA and uPAR mRNA in HCC were very significantly higher than those in C and in PTT（all P lt;0.01）；there had no significant difference between PTT and C (all P gt;0.05).There were significant correlation between the expression of CB mRNA and uPA mRNA（r＝0.805），CB mRNA and uPAR mRNA（r＝0.786），uPA mRNA and uPAR mRNA（r＝0.874）（all P lt;0.01）. Conclusion: The CB mRNA，uPA mRNA and uPAR mRNA show strongly expression in HCC and they may induce each other, promote cooperatively invasion and metastasis of HCC. Key words: hep