FAK及MMP9 蛋白在结直肠癌组织中表达及相关性探究.docVIP

FAK及MMP9 蛋白在结直肠癌组织中表达及相关性探究 　 作者：梁水权 吕益中 王羲中 王育烽 【摘要】 目的 探讨斑激酶(focal adhesion kinase , FAK)和基质金属蛋白酶9（matrix metalloproteinase，MMP9）在结直肠癌中的表达。方法 采用免疫组化法检测了67例结直肠癌和癌旁组织以及51例正常结直肠黏膜组织中FAK和MMP9的表达，并比较了不同临床病理参数下FAK和MMP9的表达情况。结果 结直肠癌组织FAK阳性表达（77.6%，52/67）高于癌旁组织（50.8%，34/67）和正常结直肠黏膜组织（37.3%，19/51），结直肠癌组织中MMP9染色阳性率（73.1%，49/67）高于癌旁组织（49.3%，33/67）和正常结直肠黏膜组织（31.4%，16/51)，不同肿瘤分化、局部分期以及有无淋巴结转移者FAK和MMP9表达水平存在差异(Plt;0. 05)。结论 FAK和MMP9在结直肠癌组织高表达，且FAK和MMP9表达呈正相关，可能成为判断结直肠癌预后的指标之一。 【关键词】 结直肠肿瘤;FAK;MMP9;免疫组织化学 [Abstract] Objective To investigate the correlation and expression of focal adhesion kinase (FAK) and matrix metalloproteinase9(MMP9) in colorectal carcinoma. Methods Expression of FAK and MMP9 were detected immunohistochemically in 67 cases of colorectal carcinoma and 51 cases of normal colorectal mucosa. Results The expression of FAK was higher in colorectal carcinoma tissue (77.6%, 52/67) than that in perineoplasm tissue (50.8%, 34/67) and normal colorectal mucosa tissue (37.3%, 19/51). The expression of MMP9 was higher in colorectal carcinoma tissue (73.1%, 49/67) than that in perineoplasm tissue (49.3%, 33/67) and normal colorectal mucosa tissue (31.4%, 16/51). The expressions of FAK and MMP9 had significant differences in colorectal tissue with different differentiation, local stage and lymph node metastasis status (P≤0. 05). Conclusion FAK and MMP9 are highly expressed in colorectal carcinoma tissue. They are positively correlative, and may become prognostic factors of colorectal carcinoma. [Key words] Colorectal carcinoma; Focal adhesion kinase (FAK); Matrix Metalloproteinase9 (MMP9); Immunohistochemistry 结直肠癌目前已成为消化外科最常见的恶性肿瘤。其癌细胞可穿透肠壁形成局部播散，亦可穿破肿瘤滋养血管或淋巴系统导致全身转移［1］。腹腔播散和远处转移依赖于肿瘤细胞具有增殖、粘附和穿破基底组织的能力，癌细胞间的粘附性能调节机制目前被认为是肿瘤演进和转移的关键因素［2, 3］。近年来研究发现，整合素介导的信号传导通路在恶性肿瘤细胞远处播散转移中具有主要作用，粘着斑激酶(focal adhesion kinase,FAK) 是整合素介导的信号转导通路的中心分子，被活化后可显著增强cfos 和cjun 等原癌基因的表达，进而促进肿瘤细胞演进、增殖、促进新生血管形成和介导肿瘤细胞与胞外基质(extracellular matrix,ECM)的粘附［4～6］。基质金属蛋白酶9（matrix me