VEGF-C对肝门部胆管癌细胞增殖以及侵袭能力影响.docVIP

VEGF-C对肝门部胆管癌细胞增殖以及侵袭能力影响 　【摘要】 　　目的：探讨血管内皮生长因子-C（VEGF-C）对肝门部胆管癌细胞侵袭和增殖的影响。方法：采用MTT法测定VEGF-C对肝门部胆管癌细胞系（FRH0201）细胞增殖的影响，流式细胞仪观察VEGF-C抗凋亡作用，应用3H-TdR 掺入试验测定VEGF-C对FRH0201细胞同质黏附作用以及Boyden小室观察VEGF-C诱导肝门部胆管癌细胞转移的作用。结果：MTT法示VEGF-C能显著提高FRH0201的增殖活性，其效应随VEGF-C剂量以及时间增加而增加，并显著抑制细胞凋亡。在Boyden小室培养的FRH0201细胞中分别加入1、5和10 ng/ml的 VEGF-C 刺激培养后， Boyden小室碳酸酯滤膜下层浸润的胆管癌细胞数均高于对照组。分别用上述浓度的VEGF-C诱导FRH0201 60、90和120 min后，FRH0201细胞的黏附能力显著低于对照组。结论：VEGF-C可以促进胆管癌细胞增殖，抑制其凋亡，增强细胞侵袭能力，并且与降低细胞的同质黏附作用密切相关。 【关键词】 血管内皮生长因子C　胆管肿瘤　细胞增殖　肿瘤转移　细胞黏附 　　Effect of vascular endothelial growth factor-C on 【ABSTRACT】 Objective: To investigate the effect of vascular endothelial growth factor-C (VEGF-C) on invasive capability and proliferation of human cholangiocarcinoma cells. Methods: The effect of VEGF-C on FRH0201 was assayed by MTT. The cycle pattern and apoptosis was assayed using flow cytometry. The effect of VEGF-C on homotypic adhesion and metastasis in FRH0201 was examined with 3H-TdR infiltration and Boyden chamber. Results: VEGF-C could enhance the proliferation of FRH0201 in a dose and time dependent manner. And VEGF-C could inhibit cell apoptosis significantly. After cultured for 2 hours with 1, 5, 10ng/ml of VEGF-C, there were more cells in the lower chamber than the control group. After 60, 90, 120 minutes induction by 1ng/ml、5ng/ml、10ng/ml, the cells showed significantly lower homotypic adhesion than that of the control group. Conclusions: VEGF-C could enhance the proliferation of FRH0201 and inhibit cell apoptosis. VEGF-C could decrease homotypic adhesion of FRH0201 and might be a cause of the metastasis. 【KＥＹ WORDS】 Vascular endothelial growth factor C·Bile duct neoplasms·Cell Proliferation·Neoplasm metastasis·Cell adhesion 　　肝门部胆管癌是一种区域性疾病，具有沿神经周围淋巴间隙以“跳跃”形式转移的特点，但其转移复发的机制尚未完全明确［1］。而血管内皮生长因子-C（vascular endothelial growth factor-C, VEGF-C）在调节肿瘤血管新生以及淋巴管生成，促进肿瘤细胞进入淋巴管，通过淋巴系统向全身转移中具有重要的作用［2］。本研究以胆管癌细胞系FRH0201为研究对象，通过观察VEGF-C对其增殖、侵袭以及黏附作用的影响，进一步研究肝门部胆管癌细胞的侵袭转移机制。 1　材料与方法 1.1　材料　人肝门部胆管癌细胞株FRH0201由