VEGF在大鼠缺血再灌注损伤肝组织中表达及其意义.docVIP

VEGF在大鼠缺血再灌注损伤肝组织中表达及其意义 　【摘要】 目的： 探讨血管内皮生长因子（vascular endothelial growth factor，VEGF）在缺血再灌注损伤肝组织中的表达及意义。方法： 利用雄性SD大鼠建立部分肝血流阻断动物模型，肝血流阻断90 min后恢复血流灌注，再灌注后1,6 h处死大鼠收集标本。分别采用全自动生化分析仪、HE 染色，比色法、ELISA法测定血清转氨酶(ALT,AST)，肝组织病理改变,肝组织髓过氧化物酶（MPO）的活性以及VEGF蛋白的含量。结果： 与假手术组相比，缺血再灌注组（I/R组）缺血肝脏MPO活性，肝组织内VEGF蛋白的含量以及ALT，AST在再灌注1 h即升高，且缺血再灌注6 h时较1 h明显升高；缺血肝脏病理切片可见大量炎症细胞浸润，6 h炎症反应更为剧烈，部分肝细胞呈点状坏死。结论： VEGF蛋白表达的上调可能参与了大鼠肝脏缺血再灌注过程中肝脏的损伤。 【关键词】 肝脏； 缺血； 再灌注损伤； 血管内皮生长因子 　　［Abstract］ Objective: To investigate the expression of vascular endothelial growth factor(VEGF) in the liver ischemia/reperfusion injury and significance.Methods： The male SD rats were used to establish partial hepatic vascular exclusion animal model. After ischemia for 90 min，SD rats liver were reperfused，and then liver specimens were collected at 1 h and 6 h after reperfusion respectively. Routine assays were performed for testing the levels of alanine aminotransferase(ALT),aspartate aminotransferase（AST）and the activity of hepatic myeloperoxidase(MPO).The changes of VEGF protein in ischemic liver lobes were determined by ELISA. The liver tissues pathological changes were evaluated in hematoxylin and eosin(HE) stained. Results： After 90 min of partial liver ischemia,the AST,ALT level in I/R group was significantly increased as compared with the sham-operated(SH) group, and AST,ALT level at the 6 h reperfusion was markly higher than that at the 1 h reperfusion.The expression of VEGF protein and the MPO activity in hepatic tissues were remarkably up-regulated in I/R group than in the SH group at the time point of 1 h and 6 h reperfusion.They were higher at 6 h reperfusion than that at 1 h. For HE staining, the infiltration of many inflammatory cells was observed in liver tissue and became much severer at 6 h posT-reperfusion liver tissue. Conclusion： The up-regulation of VEGF protein in hepatic I/R injury process might be involved in the process of liver injury. 　　［Key words］ liver； ischemia； reperfusion injury； vascular en