便秘大鼠肠壁内一氧化氮合酶表达及肠嗜铬细胞改变可复性探究.docVIP

便秘大鼠肠壁内一氧化氮合酶表达及肠嗜铬细胞改变可复性探究 　 作者：赵士彭 桂林 卞红磊 张燕 赵发 王莹 【摘要】 目的 观察便秘模型大鼠回肠和结肠一氧化氮合酶和肠嗜铬细胞的变化及自然恢复情况，了解大黄所致便秘的可恢复性。方法 Wistar大鼠60只，按照完全随机的方法分为便秘组、恢复组、对照组，便秘组和恢复组给予大黄建立便秘大鼠模型，恢复组于建立模型完成后任其自然恢复6周。以放射免疫方法和免疫组织化学方法测定比较各组大鼠回肠和结肠一氧化氮合酶和肠嗜铬细胞的变化。结果 便秘组和恢复组大鼠的回肠、结肠NOS含量均明显升高（Plt;0.05）,恢复组回肠NOS含量明显低于便秘组（Plt;0.05），恢复组结肠NOS含量与便秘组比较无差别（Pgt;0.05）。便秘组大鼠肠道5-HT免疫反应阳性的肠嗜铬细胞密度高于对照组，(回肠、横结肠Plt;0.05)。恢复组大鼠肠道5-HT免疫反应阳性的肠嗜铬细胞密度低于便秘组(回肠、横结肠Plt;0.05)但高于对照组 (回肠、横结肠Plt;0.05)。结论 长期应用大黄等蒽醌类泻剂对肠道神经系统有明显的损害作用，回肠和结肠NOS含量和肠嗜铬细胞密度升高，停用大黄恢复性饲养6周后肠道神经递质部分恢复。 【关键词】 一氧化氮合酶;肠嗜铬细胞;便秘;结肠 Alteration and recovery of nitricoxide synthase and enterochromaffin cell expression in the intestinal wall of constipation model rats ［Abstract］ Objective This study was designed to evaluate the changes of nitricoxide synthase (NOS) and enterochromaffin cell (EC) in the ileum and colon of constipated Wister rats and to observe the possibility of natural recovery. Methods Sixty healthy Wister rats were divided randomly into control group, constipation group and recovery group. In constipation group and recovery group, the rats were administered leachate of rhubarb to make constipation model. The recovery group was recovered naturally for six weeks. NOS and EC in the ileum and colon were detected by the method of immunohistochemical stain and radioimmunoassay and compared in the three groups. Results NOS expression in the constipation group and recovery group were significantly higher than that in the control group both in the ileum and in the colon (Plt;0.05). Compared with constipation group, the NOS expression in the recovery group was decreased in the ileum (Plt;0.05) and had no alternation in the colon (Pgt;0.05). Compared with control group, enterochromaffin cell density in the constipation group and recovery group was increased both in the ileum and colon (Plt;0.05). enterochromaffin cell density in the recovery group is lower than that in the constipation group both in the ileum and colon(Plt;0.05). Conclusion Long-