全流量大鼠体外循环模型建立.docVIP

全流量大鼠体外循环模型建立 　 作者：朱斌 Mihai V. Podgoreanu， G. Burkhard Mackensen， 叶铁虎 黄宇光 【摘要】 目的 建立可存活全流量的大鼠体外循环(cardiopulmonary bypass,CPB)模型。方法 8只雄性Sprague-Dawley 大鼠异氟烷吸入诱导麻醉后，经口气管内插管，接呼吸机行机械通气。进行尾动脉、腹壁尾侧浅动静脉和右侧颈外静脉血管穿刺置管等外科准备。经颈外静脉置入的多孔套管为静脉引流通道，以经尾动脉置入套管为CPB动脉灌注管道，血液经过特制膜氧合器进行氧合建立CPB并进行90 min CPB灌注。实验动物均进行血气和血流动力学等基本生理指标监测，并在CPB前、CPB后60 min以及CPB后1周测定细胞因子(IL-6和IL-10)以了解CPB所致的大鼠全身炎症情况。结果 成功建立了不输血全流量大鼠CPB模型，所有实验动物全部存活至7天以上。受试动物IL-6和IL-10在CPB后显著升高，一周后回降到术前正常水平。结论 本实验成功建立了全流量大鼠CPB模型，CPB后大鼠出现明显的全身炎症反应，这为研究CPB并发症病理生理机制以及相应的应对策略提供可靠经济的动物模型。 【关键词】 体外循环;大鼠;模型 　　Abstract: OBJECTIVE To establish a recovery rat model of full-flow cardiopulmonary bypass (CPB). METHODS Eight male Sprague-Dawley rats were used. All subjects received isoflurane inhalation anesthesia, transoral tracheal intubation and mechanic ventilation. Surgical preparations primarily involved with cannulation of four vessels including tail arteries, superficial caudal epigastric arteries and veins, and right jugular veins. The multiorificed catheter cannulated in jugular vein was used for venous drainage; the 20 guage catheter cannulated in tail artery was used for the arterial inflow from the CPB circuit and blood was oxygenated with a customized mini-oxygenator. Ninety minutes cardiopulmonary bypass was performed on the rats. Blood gas analysis and basic physiological measurements were monitored. Plasma cytokines (IL-6 and IL-10) were measured before CPB, 60 minutes after CPB and 1 week after CPB, in order to observe the CPB-induced systematic inflammation. RESULTS CPB was successfully established and all subjects were recovered from surgery and CPB and survived more than seven observation days. The plasma concentration of IL-6 and IL-10 was significantly increased after CPB but returned to the previous normal level in 7 days postoperatively. CONCLUSION A recovery rat model of full-flow cardiopulmonary bypass was successfully established. Systematic inflammation was expectedly induced by CPB, which therefore, presented a