孕激素受体亚型在子宫内膜异位症中表达及意义.docVIP

孕激素受体亚型在子宫内膜异位症中表达及意义 　 作者：奚庆华，张玉泉，徐云钊，叶青 【摘要】 目的：研究孕激素受体（PR-A+B）和孕激素受体亚型（PR-B）mRNA在子宫内膜异位症异位内膜和在位内膜中的表达及意义。方法：分别选取子宫内膜异位症患者异位内膜组织40例、在位内膜组织18例和对照组正常内膜组织20例作为研究对象，应用半定量逆转录聚合酶链反应技术（RT-PCR）检测PR-A+B mRNA,PR-B mRNA的表达。结果：PR-A+B mRNA在异位内膜的表达强度0.09±0.02明显低于在位内膜的0.81±0.16和正常内膜的0.83±0.11（P﹤0.01）；PR-B mRNA在异位内膜的表达强度0.04±0.01明显低于在位内膜的0.64±0.16和正常子宫内膜的0.63±0.09（P﹤0.01）。结论：子宫内膜异位症患者异位内膜组织中PR-A+B mRNA,PR-B mRNA的表达降低可能与子宫内膜异位症的发生发展有关。 【关键词】 子宫内膜异位症；孕激素；受体 Expression and significance of progesterone receptor isoforms in endometriosis [Abstract] Objective：To investigate the expression of progesterone receptor isoforms in the eutopic and ectopic endometrium from women of endometriosis. Methods: Forty women with endometriosis were selected as study group. 40 ectopic endometrium,and 18 matched eutopic endometrium were collected from them simultaneously at the time of surgery.20 normal endometrium were obtained as control group.The mRNA expression of progesterone receptor isoforms was detected by Reverse transcript polymerase chain reaction. Results：The density of PR-A+B mRNA expression in ectopic endometrium(0.09±0.02) from endometriosis patients was significantly fewer, compared with that in eutopic endometrium(0.81±0.16) and normal endometrium(0.83±0.11) without endometriosis（P﹤0.01）. The density of PR-B mRNA expression in ectopic endometrium(0.04±0.01) from endometriosis patients was significantly fewer, compared with that in eutopic endometrium(0.64±0.16) and normal endometrium(0.63±0.09) without endometriosis（P﹤0.01）. Conclusion:The low expression of PR-A+B mRNA and PR-B mRNA of [Key words] Endometriosis；Progesterone；Receptor 子宫内膜异位症是雌激素依赖的疾病,但一些研究发现患者的雌激素水平并无显著改变而表现为异位灶局部雌激素合成增加而灭活减少的特性。孕激素通过孕激素受体调节17β羟类固醇脱氢酶灭活雌二醇的作用， PR异常可间接影响雌激素的代谢，PR分为两种受体亚型PR-A、PR-B，本研究采用半定量逆转录聚合酶链反应技术（RT-PCR）检测子宫内膜异位症患者异位内膜和在位内膜及正常子宫内膜组织中孕激素受体PR-A+B mRNA及亚型的基因表达（PR-B mRNA），探讨孕激素受体亚型在子宫内膜异位症发病中的作用。 1 对象和方法 1.1 临床资料 收集2006年4月～2007年4月子宫内膜异位症患者40例，年龄23～45岁。按1996年美国生育学会修订的子宫内膜异位症分期法（r-AFS）进行分期，Ⅰ~Ⅱ期24例，Ⅲ期1