高纯度积雪草总苷的制备工艺研究.PDFVIP

·504 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 4 期 2014 年 2 月 高纯度积雪草总苷的制备工艺研究 陈 俊 1, 2, 3，张小龙 1, 2，杨 时 1, 2，原少伟 1, 2，丁 岗1, 2, 3，王振中 1, 2, 3，萧 伟 1, 2, 3* 1. 江苏康缘药业股份有限公司，江苏 连云港 222001 2. 中药制药过程新技术国家重点实验室，江苏 连云港 222001 3. 江苏省企业院士工作站，江苏 连云港 222001 摘 要：目的 研究高纯度积雪草总苷的制备工艺。方法 采用大孔树脂分离技术与重结晶技术相结合的方法，优选最佳积 雪草总苷制备工艺参数，同时建立积雪草总苷 HPLC 检测方法。结果 积雪草药材采用 75%乙醇回流提取、回收乙醇、水 沉、酸沉，通过 HPD-100 大孔树脂柱富集后，再采用醋酸乙酯重结晶方法，可制备质量分数 90% 以上的积雪草总苷。结论 该 工艺稳定性高、重复性好，适合于制备高纯度的积雪草总苷。 关键词：积雪草；积雪草总苷；积雪草苷；羟基积雪草苷；积雪草苷 B ；HPLC 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2014)04 - 0504 - 05 DOI: 10.7501/j.issn.0253-2670.2014.04.010 Preparing technology of asiaticosides with high-purity CHEN Jun1, 2, 3, ZHANG Xiao-long1, 2, YANG Shi1, 2, YUAN Shao-wei1, 2, DING Gang1, 2, 3, WANG Zhen-zhong1, 2, 3, XIAO Wei1, 2, 3 1. Jiangsu Kanion Parmaceutical Co., Ltd., Lianyungang 222001, China 2. State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China 3. Jiangsu Enterprise Academician Workstation, Lianyungang 222001, China Abstract: Objective To study the preparing technology of asiaticosides with high purity. Methods Macroreticular resin column chromatography and recrystallization were used to optimize the preparing technology of asiaticosides. HPLC method was established for the contcent determination of asiaticosides. Results Centella asiatica was subjected to 75% ethanol distilment, water and acid precipitation, HPD100 macroreticular resin separation, and recrystallization in acetic ester. Then asiaticosides with high purity over 90% were obtained. Conclusion The process is stable and feasible, and it could be used for the preparation of high-purity asiaticosides. Key words: Centella asiatica (L.) Urban; asiaticosides; asiaticoside; madecassoside; asiaticoside B; HPLC 积雪草 Centella asiatica (L.) Urban 为伞形科积 炎、流行性脑脊髓膜