the protection of selenium against cadmium-induced cytotoxicity via the heat shock protein pathway in chicken splenic lymphocytes硒的保护与cadmium-induced细胞毒性通过在鸡脾淋巴细胞热休克蛋白通路.pdfVIP

Molecules 2012, 17, 14565-14572; doi:10.3390/molecules171214565 OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Protection of Selenium against Cadmium-Induced Cytotoxicity via the Heat Shock Protein Pathway in Chicken Splenic Lymphocytes Xi Chen, Yi-Hao Zhu, Xin-Yue Cheng, Zi-Wei Zhang * and Shi-Wen Xu * Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China * Authors to whom correspondence should be addressed; E-Mails: zhangziwe@ (Z.-W.Z.); shiwenxu@ (S.-W.X.); Tel.: +86-451-5519-0407. Received: 9 November 2012; in revised form: 3 December 2012 / Accepted: 5 December 2012 / Published: 7 December 2012 Abstract: Cadmium (Cd) is a heavy metal that poses a hazard to animal health due to its toxicity. Selenium (Se) is an important nutritional trace element. However, the potential protective effects of Se against Cd-induced toxicity remain to be elucidated. To investigate the cytotoxicity of Cd on bird immunocytes in vitro and the protective effects of Se against exposure to Cd, chicken splenic lymphocytes received Cd (10−6 mol/L), Se (10−7 mol/L), and the mixture of 10−7 mol/L Se and 10−6 mol/L Cd and were incubated for 12 h, 24 h, 36 h, 48 h, respectively. The transcription of heat shock protein (HSP) 27, HSP40, HSP60, HSP70 and HSP90 mRNA was tested by fluorescence quantitative PCR. The results showed that the mRNA expression of HSPs exposed to 10−6 mol/L Cd showed a sustained decrease at 12–48 h exposure. A statistically significant increase in the mRNA expression