the qsec adrenergic signaling cascade in enterohemorrhagic e. coli (ehec)的qsec肾上腺素的信号级联的肠出血性大肠杆菌(ehec).pdfVIP

The QseC Adrenergic Signaling Cascade in Enterohemorrhagic E. coli (EHEC) 1,2 1,2 3 1,4 1,2 David T. Hughes , Marcie B. Clarke , Kaneyoshi Yamamoto , David A. Rasko *, Vanessa Sperandio * 1 Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 2 Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, 3 Department of Agricultural Chemistry, Kinki University, Nakamachi, Nara, Japan, 4 Institute for Genome Sciences Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, United States of America Abstract The ability to respond to stress is at the core of an organism’s survival. The hormones epinephrine and norepinephrine play a central role in stress responses in mammals, which require the synchronized interaction of the whole neuroendocrine system. Mammalian adrenergic receptors are G-coupled protein receptors (GPCRs); bacteria, however, sense these hormones through histidine sensor kinases (HKs). HKs autophosphorylate in response to signals and transfer this phosphate to response regulators (RRs). Two bacterial adrenergic receptors have been identified in EHEC, QseC and QseE, with QseE being downstream of QseC in this signaling cascade. Here we mapped the QseC signaling cascade in the deadly pathogen enterohemorrhagic E. coli (EHEC), which exploits this signaling system to promote disease. Through QseC, EHEC activates expression of metabolic, virulence and stress response genes, synchronizing the cell response to these stress hormone