the qki-6 rna binding protein localizes with the mbp mrnas in stress granules of glial cellsqki-6 rna结合蛋白定位和mbp mrna在胶质细胞压力颗粒.pdfVIP

The QKI-6 RNA Binding Protein Localizes with the MBP mRNAs in Stress Granules of Glial Cells 1 ` 1¤ 2 1 2 Yunling Wang , Genevieve Lacroix , Jeffery Haines , Evgueni Doukhanine , Guillermina Almazan , ´ 1* Stephane Richard 1Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research and Departments of Oncology and Medicine, Bloomfield Center for Research on Aging, ´ ´ ´ ´ McGill University, Montreal, Quebec, Canada, 2 Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada Abstract v Background: The quaking viable (qk ) mouse has several developmental defects that result in rapid tremors in the hind limbs. The qkI gene expresses three major alternatively spliced mRNAs (5, 6 and 7 kb) that encode the QKI-5, QKI-6 and QKI- 7 RNA binding proteins that differ in their C-terminal 30 amino acids. The QKI isoforms are known to regulate RNA metabolism within oligodendrocytes, however, little is known about their roles during cellular stress. Methodology/Principal Findings: In this study, we report an interaction between the QKI-6 isoform and a component of the RNA induced silencing complex (RISC), argonaute 2 (Ago2). We show in glial cells that QKI-6 co-localizes with Ago2 and the myelin basic protein mRNA in cytoplasmic stress granules. Conclusions: Our findings define the QKI isoforms as Ago2-interacting proteins. We also identify the QKI-6 isoform as a new component of stress granules in glial cells. Citation: Wang Y, Lacroix G, Haines