the revolution in viral genomics as exemplified by the bioinformatic analysis of human adenoviruses病毒基因组学革命以人类腺病毒的生物信息学分析.pdfVIP

Viruses 2010, 2, 1367-1381; doi:10.3390/v2071367 OPEN ACCESS viruses ISSN 1999-4915 /journal/viruses Review The Revolution in Viral Genomics as Exemplified by the Bioinformatic Analysis of Human Adenoviruses Sarah Torres 1, James Chodosh 2, Donald Seto 3 and Morris S. Jones 1,* 1 Clinical Investigation Facility, David Grant USAF Medical Center, Travis AFB, CA 94535, USA; E-Mail: sarah.torres1@ 2 Department of Ophthalmology, Howe Laboratory, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, 02114 MA, USA; E-Mail: James_Chodosh@ 3 Department of Bioinformatics and Computational Biology, George Mason University, Manassas, VA 20110, USA; E-Mail: dseto@ * Author to whom correspondence should be addressed; E-Mail: drmorrisj@; Tel.: +1-415-279-1869; Fax: +1-707-423-7267. Received: 27 May 2010 / Accepted: 24 June 2010 / Published: 28 June 2010 Abstract: Over the past 30 years, genomic and bioinformatic analysis of human adenoviruses has been achieved using a variety of DNA sequencing methods; initially with the use of restriction enzymes and more currently with the use of the GS FLX pyrosequencing technology. Following the conception of DNA sequencing in the 1970s, analysis of adenoviruses has evolved from 100 base pair mRNA fragments to entire genomes. Comparative genomics of adenoviruses made its debut in 1984 when nucleotides and amino acids of coding sequences within the hexon genes of two human adenoviruses (HAdV), HAdV–C2 and HAdV–C5, were compar