the rna editing pattern of cox2 mrna is affected by point mutations in plant mitochondriacox2信使rna的rna编辑模式是点突变影响植物线粒体.pdfVIP

The RNA Editing Pattern of cox2 mRNA Is Affected by Point Mutations in Plant Mitochondria ˆ ¤ Benoıt Castandet , Alejandro Araya* ´ ´ ´ ´ Laboratoire de Microbiologie Cellulaire et Moleculaire et Pathogenicite (M.C.M.P.- UMR5234). Centre National de la Recherche Scientifique and Universite Bordeaux Segalen. Bordeaux, France Abstract The mitochondrial transcriptome from land plants undergoes hundreds of specific C-to-U changes by RNA editing. These events are important since most of them occur in the coding region of mRNAs. One challenging question is to understand the mechanism of recognition of a selected C residue (editing sites) on the transcript. It has been reported that a short region surrounding the target C forms the cis-recognition elements, but individual residues on it do not play similar roles for the different editing sites. Here, we studied the role of the 21 and + 1 nucleotide in wheat cox2 editing site recognition using an in organello approach. We found that four different recognition patterns can be distinguished: (a) + 1 dependency, (b) 21 dependency, (c) + 1/21 dependency, and (d) no dependency on nearest neighbor residues. A striking observation was that whereas a 23 nt cis region is necessary for editing, some mutants affect the editing efficiency of unmodified distant sites. As a rule, mutations or pre-edited variants of the transcript have an impact on the complete set of editing targets. When some Cs were changed into Us, the remaining editing sites presented a higher efficiency of C-to-U conversion than in wild type mRNA. Our data suggest that the complex response observed for cox2 mRNA may be a consequence of the fate of the transcript during m