原创力文档the rna polymerase pb2 subunit of influenza ahongkong1561997 (h5n1) restrict the replication of reassortant ribonucleoprotein complexesrna聚合酶pb2亚单位流感ahongkong1561997(h5n1)限制复制重组核糖核蛋白复合物.pdfVIP
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the rna polymerase pb2 subunit of influenza ahongkong1561997 (h5n1) restrict the replication of reassortant ribonucleoprotein complexesrna聚合酶pb2亚单位流感ahongkong1561997(h5n1)限制复制重组核糖核蛋白复合物
The RNA Polymerase PB2 Subunit of Influenza A/
HongKong/156/1997 (H5N1) Restrict the Replication of
Reassortant Ribonucleoprotein Complexes
Yoko Nakazono, Koyu Hara*, Takahito Kashiwagi, Nobuyuki Hamada, Hiroshi Watanabe
Division of Infectious Diseases, Department of Infectious Medicine, Kurume University School of Medicine, Fukuoka, Japan
Abstract
Background: Genetic reassortment plays a critical role in the generation of pandemic strains of influenza virus. The influenza
virus RNA polymerase, composed of PB1, PB2 and PA subunits, has been suggested to influence the efficiency of genetic
reassortment. However, the role of the RNA polymerase in the genetic reassortment is not well understood.
Methodology/Principal Findings: Here, we reconstituted reassortant ribonucleoprotein (RNP) complexes, and demon-
strated that the PB2 subunit of A/HongKong/156/1997 (H5N1) [HK PB2] dramatically reduced the synthesis of mRNA, cRNA
and vRNA when introduced into the polymerase of other influenza strains of H1N1 or H3N2. The HK PB2 had no significant
effect on the assembly of the polymerase trimeric complex, or on promoter binding activity or replication initiation activity
in vitro. However, the HK PB2 was found to remarkably impair the accumulation of RNP. This impaired accumulation and
activity of RNP was fully restored when four amino acids at position 108, 508, 524 and 627 of the HK PB2 were mutated.
Conclusions/Significance: Overall, we suggest that the PB2 subunit of influenza polymerase might play an important role
for the replication of reassortant ribonucleoprotein complexes.
Citation: Nakazono Y, Hara K, Kashiwagi T, Hamada N, Watanabe H (2012) The RNA Polymerase PB2 Subunit of Influenza A/HongKong/156/1997 (H5N1) Restrict
the Replication of Reassortant Ribonucleoprotein Complexes. PLoS ONE 7(2): e32634. doi:10.1371/journal.pone.0032634
Editor: Earl G. Brown, Univ
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