the rph2 gene is responsible for high level resistance to phosphine in independent field strains of rhyzopertha dominicarph2基因负责独立领域的高水平耐磷化氢rhyzopertha多米尼加的菌株.pdfVIP

The rph2 Gene Is Responsible for High Level Resistance to Phosphine in Independent Field Strains of Rhyzopertha dominica 1,2 3,4 3,4 3,4 1 Yosep S. Mau , Patrick J. Collins , Gregory J. Daglish , Manoj K. Nayak , Paul R. Ebert * 1 School of Integrative Biology, The University of Queensland, Saint Lucia, Queensland, Australia, 2 Faculty of Agriculture, The University of Nusa Cendana, Kupang, Nusa Tenggara Timur, Indonesia, 3 Department of Employment, Economic Development and Innovation, Ecosciences Precinct, Brisbane, Queensland, Australia, 4 Cooperative Research Centre for National Plant Biosecurity, Bruce, Australian Capital Territory, Australia Abstract The lesser grain borer Rhyzopertha dominica (F.) is one of the most destructive insect pests of stored grain. This pest has been controlled successfully by fumigation with phosphine for the last several decades, though strong resistance to phosphine in many countries has raised concern about the long term usefulness of this control method. Previous genetic analysis of strongly resistant (SR) R. dominica from three widely geographically dispersed regions of Australia, Queensland (SRQLD), New South Wales (SRNSW) and South Australia (SRSA), revealed a resistance allele in the rph1 gene in all three strains. The present study confirms that the rph1 gene contributes to resistance in a fourth strongly resistant strain, SR2QLD, also from Queensland. The previously described rph2 gene, which interacts synergistically with rph1 gene, confers strong resistance on SRQLD and SRNSW. We now provide strong circumstantial evidence that weak alleles of rph2, together with rph1, contribute to the strong resistance phenotypes of SRSA and SR2QLD. To test