原创力文档the study on newly developed mcab nj001 specific to non-small cell lung cancer and its biological characteristics对新开发的单克隆抗体的研究nj001针对非小细胞肺癌及其生物学特性.pdfVIP
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the study on newly developed mcab nj001 specific to non-small cell lung cancer and its biological characteristics对新开发的单克隆抗体的研究nj001针对非小细胞肺癌及其生物学特性
The Study on Newly Developed McAb NJ001 Specific to
Non-Small Cell Lung Cancer and Its Biological
Characteristics
1,2 . 1,2. 1,2. 1,2 1,2 1,2 1,2
Shiyang Pan * , Fang Wang , Peijun Huang , Ting Xu , Lixia Zhang , Jian Xu , Qing Li ,
1,2 1,2 1,2 1,2 1,2 3 4
Wenying Xia , Ruihong Sun , Lei Huang , Ying Peng , Xuejun Qin , Yongqian Shu , Zhibin Hu ,
Hongbing Shen4
1 Department of Laboratory Medicine, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China, 2 National Key Clinical Department of Laboratory
Medicine, Nanjing, China, 3 Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, China, 4 Department of Epidemiology and
Biostatistics, Cancer Center of Nanjing Medical University, Nanjing, China
Abstract
Monoclonal antibody (McAb) is the key tool for cancer immunodiagnosis and immunotherapy. McAb-based
immunotherapy that targets tumor antigens has had great achivement. In this study, a cell clone which kept secreting
high-titer IgG1-type McAb named NJ001 against human non-small cell lung cancer (NSCLC) cells was obtained. The titer of
purified NJ001 was 2 6106. The antigen named SP70 of NSCLC specifically identified by NJ001 was proved to be a protein
with the relative molecular mass (Mr) of 70 kDa. The results of immunohistochemical staining indicated that NJ001 could
positively react to NSCLC, but weak positively or negatively react to human small-cell lung cance
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