无能t 淋巴细胞的建立及其免疫生物学特性的研究(Establishment of an incompetent T lymphocyte and study of its biological characteristics).doc

无能t 淋巴细胞的建立及其免疫生物学特性的研究(Establishment of an incompetent T lymphocyte and study of its biological characteristics).doc

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无能t 淋巴细胞的建立及其免疫生物学特性的研究(Establishment of an incompetent T lymphocyte and study of its biological characteristics)

无能t 淋巴细胞的建立及其免疫生物学特性的研究(Establishment of an incompetent T lymphocyte and study of its biological characteristics) The establishment of anergy of T lymphocytes and immune biological properties of.Txt as the day ideal setting for a couple in love, money under. Leaves the leaves, because the pursuit of the wind, or the tree does not retain? Kill the panda. Im the national treasure! Dont talk to me about ideals, quit! Establishment of an incompetent T lymphocyte and study of its biological characteristics [Abstract] Objective To investigate the conditions that induce the anergy of T lymphocytes in vitro and to analyze the immune biological characteristics of the incompetent T lymphocytes. Methods Wi star rat spleen cells as stimulator cells, spleen cells of SD rats as the reaction cells were mixed lymphocyte reaction (MLR) respectively, alone or in combination with dose of anti B7 21 monoclonal antibody (ranging from anti B7 21 antibody), anti B7 22 monoclonal antibody (anti B7 monoclonal antibody preparation, 22) preparation of anergy of T cells. The inability of T lymphocytes and stimulate the cells were added with anti CD3 monoclonal antibody, concanavalin A (Con A) and interleukin 2 (IL22), analysis the proliferation of T cell anergy under various conditions; will stimulate allogeneic SD cells and rat spleen cells were mixed, respectively adding different amount of anergy of T cells analysis, the proliferation of SD lymphocytes in rats. The separate application of anti B7 21 monoclonal antibody or anti B7 22 monoclonal antibody inhibited the proliferation of lymphocytes is not obvious, and the combination of the two, can obviously block the proliferation of T lymphocytes; Co N and A IL 22 can cause anergy of T cells proliferation, and anti CD3 monoclonal antibody can make incompetent T lymph cells proliferated; The inability of T lymphocytes to inhibit the proliferation of allogeneic T lymphocytes also has antigen specificity. Conclusion the combination of anti B7 2

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