心钠肽对猪卵母细胞成熟、受精和大熊猫精子功能影响.pdf

wasdetectedinfresh thelevelsof receptors-A(NPRA)mRNAsperm．while natriuretic Deptide werelowwithno receptors_C(NPRC)mRNAobvious different change oviductal among phases．the levelsofANPmRNAwere in and were high lowino，r2．OnIhe OTl，o，13orr5，butvery otherhand． thelevelsofNPRArnRNAwerelowin and OTl inOT3andreacheda in OT2，increased maximum OT4andOT5．neeffectofANPon functionwasstudiedthe spermatozoa by acrosomereactionand IVEIncubationwithANPfor1h inducedacrosomereactionof significantly preincubatedspermatozoa， andmaximal ofacrosome achievedat1 nMANP response reaction(34．1±2．3％)was treatment．Both selectiveof caffeinehadnoeffectontheacrosome C-ANP-(4-23)，aligandNPRC，and reaction．The effectof on ANPacrosomereactioncouldbemimickedlbe stimulatory bv cGMP permeableanalog． 8一Br-cGMEANPandcaffeinehadthesimilareffecton the rate． improvingoocytespenetration rateandthe numberof treatmentthe had polyspermy average spermperpenetratedoocytes．AIso．ANP similareffecton formationrateandthenumberofceils asthatof cleavag