低温胁迫下高羊茅抑制消减文库的构建和分析.PDFVIP

2009 11 17 6 Chinese Journal of Eco-Agriculture, Nov. 2009, 17(6): 1162−1167 DOI: 10. 3724/SP.J.1011.2009.01162 * 黄锦文 骆 娟 陈冬梅 郑红艳 林文雄** ( 350002) 采用抑制消减杂交(SSH)技术构建了高羊茅“猎狗 5 号”低温下的正向差减 cDNA 文库, 并从基因表 达水平研究了低温诱导对高羊茅抗寒性的影响。通过 Reverse northern 杂交法筛选, 挑选出 36 个低温处理下差 异表达基因, 并对这些基因进行测序。将测序结果在基因组数据库(NCBI) 中进行检索比对, 最后获得 34 个差 异表达的基因, 其中 33 个基因功能已知, 主要与植物信号转导、光合作用、糖代谢、物质运输以及抗逆性相关。 高羊茅 低温处理 抑制消减杂交 基因表达 抗寒性 : S688.4 : A : 1671-3990(2009)06-1162-06 Construction and analysis of suppression subtractive library of Festuca arundinacea to low temperature stress HUANG Jin-Wen, LUO Juan, CHEN Dong-Mei, ZHENG Hong-Yan, LIN Wen-Xiong (Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstract A forward subtractive cDNA library was constructed from tall fescue “Houndog 5” cultivar exposed to low temperature stress via suppression subtractive hybridization (SSH). Then cold-resistance of tall fescue (induced by low temperaturing) was de- termined through genes expression analysis. A total of 36 ESTs from SSH-cDNA library were selected and sequenced after reverse northern hybridization screening. The sequencing results were analyzed by means of retrieval and alignment in unigene GeneBank database. Based on the analysis, 34 genes exhibited different expressions, of which the functions of 33 genes were known and identi- fied. These functionally known genes were detected in signal transduction, photosynthesis, sugar metabolism, material transportation and stress resistance. Key words Festuca arundinacea , Low temperature treatment, Suppression subtractive hybridization (SSH), Gene expression, Cold resistance (Received March 27, 2009; accepted May 8, 2009) (Festuca arundinacea) 5 ”, (Suppression , ,