FHIT及PCNA在宫颈上皮内瘤变及宫颈癌中表达及临床意义.docVIP

FHIT及PCNA在宫颈上皮内瘤变及宫颈癌中表达及临床意义 　【摘要】 目的 研究脆性组氨酸三联体基因（FHIT）蛋白和增殖细胞核抗原（PCNA）在正常宫颈、宫颈上皮内瘤变(CIN)和宫颈癌中的表达情况，探讨两者在宫颈癌早期诊断和发生发展中的临床意义。方法 采用免疫组织化学SP法检测20例正常宫颈组织；20例宫颈CINⅠ级、25例CINⅡ/Ⅲ级；60例宫颈癌组织中的FHIT蛋白和PCNA的表达。结果 FHIT蛋白在正常宫颈组、宫颈CIN组、CINⅠ组、CINⅡ/Ⅲ组和宫颈癌组中的阴性率分别为0%（0/20）、24.4%（11/45）、10.0%（2/20）、36.0%（9/25）和66.7%（40/60），各组间阴性率比较差异有统计学意义（P＜0.05）；PCNA在正常宫颈组织、宫颈CIN组、宫颈癌组中的阳性率分别是65.0%（13/20）、93.3%（42/45）和98.3%（59/60），各组间阳性率比较差异有统计学意义(Plt;0.05)；在宫颈CIN和宫颈癌中，FHIT和PCNA的表达有一定的相关性(Plt;0.05)。结论 检测宫颈CIN中的FHIT蛋白表达情况，可用作高级别的CIN筛查或预测。FHIT和PCNA对于宫颈癌前期病变和宫颈癌的筛查和早期诊断具有一定的临床参考价值。 【关键词】 宫颈上皮内瘤变 宫颈癌 组氨酸三联体 增殖细胞核抗原 免疫组织化学 早期诊断 【Abstract】 Objective To study the expression of fragile histidine triad (FHIT) and proliferating cell nuclear antigen (PCNA) in normal cervix, cervical intraepithelial neoplasia (CIN) and cervical cancer. To discuss the potential involvement of FHIT and PCNA in tumorigenesis，metastasis in cervical cancer; to discuss the significance of FHIT and PCNA in early diagnosis of cervical cancer. Methods The expressions of FHIT protein and PCNA were detected by immunohistochemistry in 20 cases of normal cervix, 20 cases of CINⅠ,25 cases of CINⅡ/Ⅲ, 60 cases of cervical cancer. Results The percentages of negative expression of FHIT protein in normal tissues were 0%(0/20), 24.4%（11/45）in CIN group,10.0%（2/20） in CINⅠgroup,36.0%（9/25）in CINⅡ/Ⅲ group and 66.7%（40/60）in cervical cancer group. There were significant differences between groups (Chi-square test, Plt;0.05). The positive expression rate of PCNA wase 65.0% （13/20）in normal group, 93.3% （42/45）in CIN group, and 98.3%（59/60）in cervical cancer group. There was significant difference between groups (Chi-square test, Plt;0.05). FHIT protein expression and PCNA expression were negatively related (Plt;0.05) in CIN and cervical cancer. Conclusion Since the expression level of FHIT is associated with the grade of CIN, it can be used to screen or forecasting higher-grade- group of CIN. FHIT and PCNA has clinical reference value for screening