STAT3在胆囊癌组织中表达及意义.doc

STAT3在胆囊癌组织中表达及意义 　【关键词】 ,胆囊癌 摘 要：目的：通过研究STAT3基因在原发性胆囊癌组织中的表达，探讨其在胆囊癌发生发展中的作用机制。方法：免疫组织化学法检测52 例胆囊癌组织、17例癌旁组织和12 例正常胆囊组织中STAT3 蛋白表达情况。结果：STAT3蛋白在胆囊癌组织、癌旁组织和良性病变胆囊组织中的阳性率分别为69.23%（36/52）、52.94%（9/17）和25.00%(3/12)。在胆囊癌组织和癌旁组织中， STAT3蛋白的表达差异无显著性意义（Pgt;0.05）。相对于正常的胆囊组织，在胆囊癌组织和癌旁胆囊组织中，STAT3蛋白的表达率明显增加，他们之间有显著的差异（Plt;0.05）。结论：相对于正常的胆囊组织，在胆囊癌组织和癌旁胆囊组织中，STAT3蛋白的阳性率和表达强度明显增高（Plt;0.05）。提示，STAT3高表达可能是胆囊癌发病机制中的早期事件；在癌旁胆囊组织中，那些STAT3蛋白阳性的胆囊细胞可能是潜在的恶性癌前细胞。 　　关键词： 胆囊癌; STAT3; 免疫组织化学法 　　The Expression and Significance of STAT3 in Gallbladder Carcinoma Tissues 　　Abstract：Objective： By researching the expression of STAT3 in human gallbladder carcinoma tissues， we explored its role mechanism in the occurrence and development of gallbladder carcinoma. Method： The protein expressions of STAT3 were detected in 52 gallbladder carcinoma tissues, 17 para-carcinoma tissues and 12 benign gallbladder tissues by IHC.Result： Positive expression rates of STAT3 protein in gallbladder carcinoma tissues, para-carcinoma tissues and benign gallbladder tissues were 69.23%（36/52）,52.94%（9/17）and 25.00%(3/12), respectively . There was no significant difference between the expression of STAT3 protein in gallbladder carcinoma tissues and para-carcinoma tissues（Pgt;0.05）; Compared with normal gallbladder tissues, the expression of STAT3 protein in gallbladder carcinoma tissues and para-carcinoma tissues were significantly higher（Plt;0.05）. Conclusion： Compared with normal gallbladder tissues, the positive expression rate and intensity of STAT3 protein in gallbladder carcinoma tissues and para-carcinoma tissues were significantly higher（Plt;0.05）.It suggested that overexpression of STAT3 gene may be an early accidence in the pathogenesis of gallbladder carcinoma. In para-carcinoma gallbladder tissue,those STAT3-positive cells may be potential malignant pre-cancerous cells. 　　Key words： Gallbladder carcinoma； STAT3； Immunohistochemistry 　　本研究旨在探讨STAT3在胆囊癌组织、癌旁组织和正常胆囊组织中的表达及其意义。 　　1 材料与方法 　　1.1 标本来源:收集2001年10月至