Tenascin、CD34在胆管癌组织中表达及临床意义.doc

Tenascin、CD34在胆管癌组织中表达及临床意义 　【关键词】 胆管癌 摘要：目的 探讨Tenascin、CD34在胆管癌的发生、发展中的作用和意义。方法 收集我院1995年-2003年期间切除的胆管癌标本33例和正常胆管组织4例，采用免疫组织化学SP方法，检测其Tenascin、CD34的表达情况。结果 Tenascin在胆管癌组织中的阳性表达率为81.8%(27/33)，其中病理分级Ⅰ级、Ⅱ级、Ⅲ级的阳性率分别为71.4%、82.4%和88.9%，但无显著性统计学差异(Pgt;0.05)；在正常胆管组织中未检测到Tenascin表达。CD34 在胆管癌组织中广泛表达，在正常胆管组织中仅有少量表达，两者相比具有显著统计学差异(Plt;0.05)。结论 Tenascin可以作为胆管癌的肿瘤标志应用于临床诊断， CD34可作为预测肿瘤转移的一项重要指标。 　　关键词：胆管癌；Tenascin；CD34；免疫组织化学 　　Expression and clinical significance of tenascin and CD34 in human cholangiocarcinoma 　　ABSTRACT: Objecive To investigate the role of tenascin and CD34 in occurence and development of cholangiocarcinoma. Methods The expression of tenascin and CD34 were examined in 33 patients, 16 males, 17 femalse, ranged 32-70 years with cholangiocancinoma and in 4 cases of normal bile duct tissue by SP immunohisto chemical technique. Results TN expression was located in the stroma of cholangiocarcinoma. The proportion of positive TN expression was 81.8%(27/33). The positive rate of pathologic grade Ⅰ, Ⅱ and Ⅲ were 71.4%, 82.4% and 88.9%, respectively. There was no significant difference among the three graded groups. TN expression wasnt detected in normal tissues (Pgt;0.05). CD34 showed widespread expression in cholangiocarcinoma tissues, but limited in normal bile duct, which showed significant difference (Plt;0.05). Conclusion TN was over expressed in cholangiocarcinoma compared with negative expression in normal tissues, which suggested the diagnostic value of TN for cholangiocarcinoma. The levels of MVD marked by CD34 were significantly higher in cholangiocarcinoma than that in normal tissue. Whats more, there were significant correlation between MVD and pathologic grades and/or metastasis. 　　KEY WORDS: cholangiocarcinoma; tenascin; CD34; immunohistochemistry 　　胆管癌是较常见的恶性肿瘤之一，具有诊断困难、恶性度高、转移早、预后差等特点。近年来，人们发现细胞外基质(extracellular matrix, ECM)与肿瘤生长转移关系密切。Tenascin(TN)是细胞外基质中一种具有独特六臂体结构的高分子糖蛋白[1]，在成熟组织中不表达或仅有少量表达。近来研究表明，TN在许多人类恶性肿瘤组织中有较高表达[2-3]，且其表达与肿瘤病理分级和肿瘤血管生成关系密切。 CD34