大肠癌患者外周血CD4+ CD25+ CD127lo--调节性T细胞含量及临床.docVIP

大肠癌患者外周血CD4+ CD25+ CD127lo/-调节性T细胞含量及临床 　【摘要】 目的 探讨大肠癌患者外周血CD4+、CD25+、CD127lo/-调节性T细胞（Treg）的变化及意义。方法 流式细胞术检测36例大肠癌患者及30例健康人外周血中CD4+、CD25+、CD127lo/-Treg的变化；ELISA检测同一标本血清中转化生长因子β（TGF-β）的表达水平。结果 36例大肠癌患者外周血中CD4+、CD25+、CD127lo/-Treg占CD4+、淋巴细胞的比例为（6.92±1.31）%，与正常对照组（4.91±1.24）%相比差异有显著性（Plt;0.05）。20例腺癌、7例黏液癌、9例未分化癌患者外周血中CD4+、CD25+、CD127lo/-Treg比例分别为(6.81±1.52) %、(6.99±1.21) %、( 7.08±1.17) %，各组间比较差异无显著性(Pgt;0.05)，均高于对照组的(4.91±1.24)%，差异有显著性（Plt;0.05）。Ⅳ期大肠癌患者外周血CD4+、CD25+、CD127lo/-Treg 高于Ⅱ期大肠癌患者（Plt;0.05）。大肠癌组外周血清中TGF-β水平显著高于正常对照组，随着TGF-β水平增加，CD4+、CD25+、CD127lo/-Treg比例逐渐增高，两者呈正相关。结论 大肠癌患者外周血中CD4+、CD25+、CD127lo/-Treg表达增高，与临床分期相关，但与组织学分类无关，导致机体免疫抑制，可能参与大肠癌发生。 【关键词】 大肠癌 CD4+、CD25+、CD127lo/-调节性T细胞 转化生长因子β 　　【Abstract】 Objective To evaluate the changes of CD4+CD25+CD127lo/- regulatory T cells in peripheral blood from patients with colorectal carcinoma. Methods 36 patients with colorectal carcinoma and 30 healthy volunteers were included in this study. The proportion of CD4+CD25+CD127lo/- regulatory T cells population in CD4+ T cells was evaluated by flow cytometric analysis. The TGF-β production was measured by ELISA. Results Patients with colorectal carcinoma had significantly higher percentages of CD4+CD25+CD127lo/- regulatory T cells than that of healthy controls (6.92±1.31% vs 4.91±1.24%, Plt;0.05), the proportion of CD4+CD25+CD127lo/- regulatory T cells in patients with adenocarcinoma (n= 20), mucinous carcinoma (n = 7) and undifferentiated carcinoma (n = 9) were all obviously higher than that of healthy controls(Plt;0.05), but there weren’t obvious differences among the three groups of different pathological type (Pgt;0. 05).The proportion of CD4+CD25+CD127lo/- regulatory T cells in the peripheral blood of 12 colorectal carcinoma patients with Ⅳ stages significantly higher than that of 8 colorectal carcinoma patients withⅡstages (7.23±1.34% vs 6.56±1.18%). Increased level of TGF-β was also detected in colorectal carcinoma patients, there was positive correlation between TG