大鼠增龄过程中骨质疏松及椎间盘退变相关性分析.docVIP

大鼠增龄过程中骨质疏松及椎间盘退变相关性分析 　 作者：张银刚　李天清　王金堂　郭雄 【摘要】 　　目的 研究大鼠增龄过程中骨质疏松症和椎间盘退变的相关性。方法 50只健康纯种SD大鼠，喂养22个月，建立老龄大鼠模型，进行股骨、胫骨、腰椎等组织学观察，并评价椎间盘退变的相关指标(椎间盘软骨Ⅱ型和Ⅹ型胶原表达、软骨终板血管芽相对面积、软骨终板钙化层/非钙化层厚度)与骨质疏松的相关指标(骨皮质厚度指数、骨小梁相对面积、骨密度、骨最大应力和应变)的相关性。结果 骨质疏松相关指标骨皮质厚度指数、骨小梁相对面积、股骨密度、椎体骨密度与椎间盘退变指标终板血管芽相对面积、非钙化/钙化层比值、Ⅱ型胶原定量之间存在中度到强度的负相关，而与Ⅹ型胶原表达存在中度到强度的正相关，其中椎体骨密度与软骨终板的Ⅹ型胶原相关性最强。骨生物力学指标最大变形和最大负荷与椎间盘退变的各个指标无相关性。结论 骨质疏松和椎间盘退变呈负相关，这种相关并不能完全归因于环境因素；而骨生物力学最大变形、最大负荷和椎间盘退变无相关性。 【关键词】 骨质疏松症；椎间盘；老龄大鼠 　　　Analysis of the correlation between osteoporosis and degeneration of intervertebral disc in the aging rat 　　　ABSTRACT: Objective To study the correlation between osteoporosis and degeneration of intervertebral disc in the aging rat. Methods Fifty healthy SD rats were fed for twentytwo months in order to build the model of aging rats, the histology of femoral bone, tibial bone and lumbal vertebra was used to observe, then we evaluated the indexes which reflect the degeneration of intervertebral disc including the quantity of collagen type Ⅱ, Ⅹ of intervertebral disc by the immunohistochemistry methods, the relative area of vascular bud, the thickness of calcified layer and uncalcified layer on cartilage endplate and the indexes which reflect the osteoporosis including the thickness ratio of cortical bone, the relative area of bone trabecula, the bone density, the max stress and straining of bone. Results There was moderate or strong negative correlation among the indexes of the degeneration of intervertebral disc including the relative area of vascular bud, the ratio of the uncalcified and the calcified layer, the quantitation of collagen type Ⅱand the indexes of osteoporosis including the thickness ratio of cortical bone, the relative area of bone trabecula, the density of femoral bone and vertebral body bone, the max stress and straining of bone. However, there was moderate or strong positive correlation with the quantitation of collagen type Ⅹ, of which the bone density of vertebral body had the