大鼠脊髓再灌注损伤中三甲氧苄嗪保护作用.docVIP

大鼠脊髓再灌注损伤中三甲氧苄嗪保护作用 　 作者：朱晓峰，史宏宇，马宝红，邹志田，龙村 【关键词】 再灌注损伤 摘要：目的 评价三甲氧苄嗪对大鼠脊髓再灌注损伤的保护作用，并探讨其作用机制。方法 45只SD大鼠随机分为3组，假手术组、对照组和三甲氧苄嗪组，每组15只。建立大鼠脊髓缺血损伤模型，假手术组不阻断主动脉，对照组在肾动脉以下及髂动脉以上同时阻断主动脉20min，三甲氧苄嗪（TMZ）组于主动脉阻断前10min静脉内注射三甲氧苄嗪（3mg/kg）,余同对照组，20min后开放主动脉。分别于阻断主动脉前、松开主动脉前和松开主动脉后2h取静脉血测定丙二醛（MDA）含量。术后48h按Tarlov评分标准评价动物后肢神经功能，然后处死动物，取脊髓进行含水量、MDA含量测定及组织病理学检查。结果 TMZ组血中MDA含量明显低于对照组（P＜0.05）；TMZ组动物后肢神经功能评分明显高于对照组（P＜0.01）；TMZ组脊髓含水量、MDA含量明显低于对照组（P＜0.01）；TMZ组光镜下脊髓病理改变轻微，而对照组较重，两组病理评分有显著性差异（P＜0.01）。结论 三甲氧苄嗪对大鼠脊髓缺血再灌注损伤具有明显的保护作用。 　　关键词：三甲氧苄嗪；脊髓；再灌注损伤 　　The Protective Effect of Trimetazidine on Reperfusion Injury of Spinal Cord in Rats 　　Abstract: OBJECTIVE To evaluate the effect of trimetazidine on reperfusion injury of spinal cord in rats and discuss its mechanism .METHODS With rat spinal cord ischemia model , we induced ischemia by clamping the aorta just distal to the renal artery and above the iliac bifurcation for 20 min . Forty-five spraque-dawley rats were randomly assigned to three groups(n=15 in each group ) . Sham-operation group subjected to operation dissections without aortic occlusion ; control group undergoing occlusion but receiving no treatment ; TMZ （trimetazidine）group receiving 3mg/kg trimetazidine intravenously in ten minutes before the occlusion of aorta. The malondialdehyde(MDA) content in blood was measured before the occlusion, during the ischemia and after 2 hours of reperfusion respectively. Neurologic status was assessed in 48h after operation , then all animal were sacrificed and the water content , MDA content of spinal cord were also measured . The spinal cord was processed for histopathologic examination . RESULTS Compared with the control group , the TMZ group had lower water content and MDA content (Plt;0.01) . The neurological scoring of TMZ group was significantly higher than that of control group (Plt;0.01) . The histopathologic change of spinal cord in TMZ group was milder compared with that in control group (Plt;0.01) . CONCLUSION Trimetazidine could