白膜法手工富集血小板及单供者机采血小板质量对比探究.docVIP

白膜法手工富集血小板及单供者机采血小板质量对比探究 　【摘要】 本研究旨在对比白膜法手工富集血小板与单供者机采血小板的质量差异。将15袋（2单位/袋，400 ml全血制备）白膜法手工富集血小板和15单位单供者机采血小板在（22 ±2 ）℃条件下振荡保存。在制备后1小时检测血小板浓度、体积、白细胞残留量、红细胞残留量；分别在血小板保存的0、1、2、3、4、5天检测平均血小板体积、pH值、乳酸浓度、血小板膜表面CD62p表达率、凝血酶激活后CD62p再表达率、血小板低渗休克反应。结果显示：二组的血小板得率、红细胞残留量、白细胞残留量都分别达到相应的国家质量标准，但在达到相同血小板计数的情况下，白膜法手工富集血小板组的红细胞残留量、白细胞残留量明显高于单供者机采血小板组（p＜0.01）；二组的乳酸、血小板膜表面CD62p表达率均随保存时间延长而升高；二组间比较，白膜法手工富集血小板组CD62p表达率在保存0-5天均高于单供者机采血小板组（p＜0.01）。二组的pH值、凝血酶激活后CD62p再表达率均随保存时间的延长而下降；二组间比较，2-5天白膜法组pH值明显低于单供者机采血小板组（p＜0.01），0-5天白膜法组CD62p再表达率明显低于单供者机采血小板组（p＜0.01）。单供者机采血小板组血小板低渗休克反应水平在0-5天无明显变化，而白膜法手工富集血小板组血小板低渗休克反应水平随保存时间的延长而下降，保存1-5天时均明显低于单供者机采血小板组（p＜0.01）。结论：白膜法手工富集血小板质量低于单供者机采血小板质量，其制备工艺有待改进和优化，以降低红细胞、白细胞残留量和活化血小板水平，提高其血小板质量。 【关键词】 单供者机采血小板 Qualitative Comparison between BuffyCoatCollected Platelet Concentrates and those by Singledonor Plateletapheresis Abstract This study was aimed to compare the difference of quality between buffycoatcollected platelet concentrates (BCPC) and singledonor plateletapheresis (SDP). 15 packs of BCPC and 15 units SDP were stored at 20℃-24℃ with agitation. Platelet concentration， platelet volume, residual leukocyte and residual erythrocyte in two groups were examined after preparation for 1 hour. Mean platelet volume, pH value, hypotonic shock response (HSR) , CD62p expression and CD62p reexpression of platelet were detected on 0, 1, 2, 3, 4, 5 days of platelet preservation. The results showed that the platelet yields, residual leukocyte and residual erythrocyte in two groups accorded with the national quality standard respectively, but residual leukocyte and residual erythrocyte in BCPC group were higher than those in SDP group when platelet yields in two groups were equal（p＜0.01）. Lactate concentration, CD62p expression of platelet increased with prolongation of preseved time, while pH value decreased gradually. Compared with SDP group, there were significant differences in CD62p expression, CD62p reexpression of platelet preserved for 0-5 days