腺性膀胱炎及膀胱移行细胞癌中MMAC1表达及意义.docVIP

腺性膀胱炎及膀胱移行细胞癌中MMAC1表达及意义 　【摘要】 ［目的］ 研究MMAC1在腺性膀胱炎和膀胱移行细胞癌中的表达，探讨其与肿瘤发生、发展的关系。［方法］ 采用免疫组化方法测定20例腺性膀胱炎和40例膀胱移行细胞癌标本中的MMAC1蛋白的表达，以10例正常膀胱黏膜作为对照。［结果］正常膀胱黏膜中MMAC1的阳性表达率高于腺性膀胱炎和膀胱移行细胞癌，MMAC1表达缺失与膀胱移行细胞癌病理分级、临床分期明显相关。［结论］ MMAC1表达缺失可能与膀胱移行细胞癌的发生、发展有关，检测MMAC1的表达将有助于评估膀胱移行细胞癌的恶性程度及预后。 【关键词】 腺性膀胱炎 膀胱移行细胞癌 免疫组织化学 MMAC1蛋白 The Expression and Significance of MMAC1 in Cystitis Glandularis and Bladder Transitional Cell Carcinoma CHEN Xiao-bo1, CHEN Jian2, TAN Jian-ming1 (1.The First Affiliated Hospital, Shanghai Jiaotong University, Shanghai 200080, China; 2.The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China) Abstract：［Purpose］To investigate the expression of mutated in multiple advanced cancer (MMAC1) in cystitis glandularis and bladder transitional cell carcinoma (BTCC), and to investigate the relationship between the expression of MMAC1 and the occurrence and development of BTCC. ［Methods］ Immunohistochemistry was used to detect the expression of MMAC1 in 20 cases with cystitis glandularis and 40 cases with BTCC, and 10 cases with normal bladder mucosa tissue were used as control. ［Results］ The positive rate of MMAC1 expression was much higher in normal bladder mucosa tissues than those in cystitis glandularis or BTCC. The MMAC1 negative expression was significantly correlated with grading and clinical staging BTCC. ［Conclusion］ MMAC1 negative expression might be related to occurrence and development in BTCC. To detect MMAC1 expression will contribute to the evaluation of the malignancy and prognosis for BTCC. Key words: cystitis glandularis; bladder transitional cell carcinoma; immunohistochemistry; MMAC1 protein 多种进展期肿瘤突变基因 (mutated in multiple advanced cancer 1，MMAC1)是至今为止发现的第一个具有磷酸酶活性的抑癌基因[1]。研究证实MMAC1基因在多种人体正常组织中高表达，而在人体增殖细胞、良性肿瘤、癌中部分或完全失活。我们采用免疫组化方法对腺性膀胱炎和膀胱移行细胞癌(bladder transitional cell carcinoma，BTCC)中MMAC1蛋白的表达进行检测，以探讨其与BTCC发生、发展的关系及其临床意义。 1 材料与方法 1.1 一般资料 标 本：采用广西医科大学第一附属医院2000年至2003年间病理活检及开放手术切除的标本，腺性膀胱炎20例，BTCC 40例，BTCC中男性33例，