细胞生物学chapter2-Research method.pdf

Outline Chapter II Research Method of Cell Biology Microscopic Imaging Technique Cytochemical Technique Cell Sorting Cell Engineering Technology Separation Technology Methods in Molecular Biology 新的助教邮箱：cytobiosjtu@126.com 助教公共网盘：百度网盘（ ） 账号：cytobiosjtu@126.com 密码：learn4fun 网盘中存放有作业规范，大家可以分享软件教程， 每次作业改完以后保存优秀的作业，供大家参考。 A Sense of scale between living cells and atoms Figure. Molecular Biology of the Cell (© Garland Science 2008) 2.1 Microscopic Imaging Technique 2.1.1 Light microscope It allows us to magnify cells up to 1000 times and to resolve details as small as 0.2 μm. Three things are required for viewing cells in it:  Light system(light and condenser)  Specimen must be prepared carefully to allow light to pass through it.  Lenses (objective and eyepiece) Figure . Essential Cell Biology (© Garland Science 2010) Figure. Molecular Biology of the Cell (© Garland Science 2008) Resolution(r. 分辨率) is the shortest distance between two separate points in a microscope’s field of view that can still be distinguished as distinct entities. Numerical aperture (数值孔径, NA): Resolution, Magnification objective The numerical aperture with respect to a point P depends on the half- angle θof the maximum cone of light that can enter or exit the lens. The numeric