血清葡萄糖测定的溶血干扰及结果校正（Hemolytic interference and correction of serum glucose determination）.docVIP

血清葡萄糖测定的溶血干扰及结果校正（Hemolytic interference and correction of serum glucose determination） Hemolytic interference and correction of serum glucose determination Update Date: 10-07 Li Jiaoqing Hexokinase method for the determination of glucose is one of the few clinical methods used in routine assays. Generally believed that for mild hemolysis, fat, blood, jaundice and other factors will not be disturbed. But when we used the Hitachi 7170 automatic biochemical analyzer of hemolysis, often found low. This paper discusses the phenomenon and compares the blood glucose measured by two different wavelengths. The hemolysis index and hemoglobin are measured, and the method of correcting the result of hemolysis sample is discussed. Materials and methods 1. Sample origin: 120 cases of fasting venous blood of outpatients in our hospital. Two reagent: 1. glucose determination (hexokinase method) kit (Human, Germany). (2) glucose standard solution: glucose 555 mmol/L (Ministry of Health * * Biological Products Research Institute). 3. hemoglobin quality control products: 128.9 g/L. Three, instrument: 1. Hitachi 7170 automatic biochemical analyzer (* * Hitachi). 2.Vitalab Selectra automatic biochemical analyzer (* * Rittal). Methods: 1. four, 120 patients used Hitachi automatic biochemical analyzer (405 nm/340 nm) determination of glucose by hexokinase, blood glucose in the range of 5 ~ 8 mmol/L, and then add the control samples containing hemoglobin, hemoglobin concentration were 0.5 g/L, 1 g/L and 1.5 g/L, using Hitachi automatic biochemical analyzer (wavelength 405 nm/340nm) and Rittal automatic biochemical analyzer (wavelength 380 nm/340nm) glucose by hexokinase method, and compare different effects on serum glucose concentration hemoglobin results, to obtain numerical compensation. 2. hemolysis index of hemoglobin samples containing different concentrations were determined. Result The concentration of glucose in 1.120 cases of different concentrations of hemoglobin serum samples