研究蛋白质间相互作用的主要技术（The main techniques for studying protein-protein interactions）.docVIP

研究蛋白质间相互作用的主要技术（The main techniques for studying protein-protein interactions） To explore the molecular mechanisms of biological processes, the protein-protein interactions that mediate this process need to be identified. The main techniques for studying protein-protein interactions are summarized below: Yeast two hybrid system Yeast two hybrid system is an important method widely used in proteomics study of protein interaction. The principle is that when combined with target protein and protein specific bait, bait protein binding to the reporter gene promoter, expression of reporter genes in yeast cells start, if detected gene expression products reported, it shows that the interaction between the two, there is no interaction between them and vice versa. This technique will be used for large-scale protein interaction studies after micro and array. In actual work, people have developed single hybrid system, three hybrid system and reverse hybridization system as needed. Angermayr et al designed a SOS protein mediated two hybrid system. We can study the function of membrane protein and enrich the function of yeast two hybrid system. In addition, the role of the yeast two hybrid system has also been extended to the identification of proteins. Two, phage display technology The DNA sequence is connected with a monoclonal antibody encoding phage coat protein gene, when phage growth, surface expression of the corresponding monoclonal antibody, then phage column, if the column containing the target protein, it will be combined with corresponding antibody specificity, which is called phage display technology. This technology is mainly used to study the interaction between proteins, not only has high throughput and simple characteristics, also has the direct and selective gene screening of complex mixtures, by changing the conditions in the screening process can be combined with specific advantages of direct assessment. At present, the cDNA Library of two specific cell lines