骨肿瘤组织OPG%2fRANKL表达对其生物学行为的影响.pdfVIP

上海交通大学医学院2005 级硕士学位论文 3 ．实验组裸鼠注射肿瘤细胞后6 周， RANKL 免疫组化染色可见 胫骨髓腔内约50 ％的骨髓基质细胞膜RANKL 染色可见强阳性反应。 研究结论 1．RANKL 、OPG 的表达量是与肿瘤组织的恶性程度相一致的。 2 ．成骨细胞与PC-3 细胞接触后能增强成骨细胞RANKL 表达，其 可能的机制是通过肿瘤细胞表面RANK 受体与成骨细胞表面RANKL 结 合后，进一步刺激成骨细胞表达更多的RANKL 。 3 ．PC-3 细胞在动物体内同样可以增强成骨细胞的 RANKL 表达， 最终导致肿瘤溶骨性破坏。 关键词：OPG，RANKL，RANK，骨肿瘤，生物学行为 2 上海交通大学医学院2005 级硕士学位论文 THE EXPRESSION OF OPG/RANKL IN BONE TUMOR TISSUE AND ITS IMPACT ON THE BIOLOGICAL BEHAVIOR OF TUMOR ABSTRACT Objective To discuss the effect of OPG/RANKL/RANK system in the onset and development of primary and secondary bone tumor, and study the possible mechanism of the system in metastasis of prostate cancer to bone, thus provide a theoretic and exprimental basis for the clinical study of osteolytic lesion. Methods 1. Using the immunohistochemistry method to study the expression of OPG/RANKL on metastatic bone tumor and bone giant cell tumor, and comparing the difference among the expression of OPG/RANKL in different tissue 2. To set up a metastatic tumor model in vitro by co-culturing the osteoblast and prostate cancer cell line PC-3 together, and compare the difference of OPG/RANKL/RANK expression on osteoblast with that of prostate cancer cell 3. To set up an animal model by intra-tibia injection, and sacrifice the animal after 2, 4, 6, 8, 10 weeks, observe the growth of the tumor, the lesion of bone tissue and RANKL expression on tumor by taking X-ray photography, H-E stain and anti-RANKL immunohistochemistry stain. Results 1. The expression of OPG is different in different tissue. OPG stain is negative in benign prostatic hyperplasia. In prostate cancer tissue and most metastatic bone tumor, the