Aβ对大鼠血管平滑肌细胞的影响及依达拉奉干预作用的研究.pdfVIP

华 中 科 技 大 学 硕 士 学 位 论 文 Part one Effect of A β on the vascular smooth muscle cells of rat Abstract Obstract : to observe the damage of A β to SMC of rat, and study the alteration of the expression of APP gene caused by A β. Method : VSMC of rat were cultured in vitro, control group: VSMC were cultured with substratum; experimental group: VSMC were cultured with A β and substratum. Morphologic changes of VSMC was observed under the microscope. The viability of VSMC was detected by MTT, The rate of apoptosis was detected by flow-cytometric examination. The expression of APP gene was detected by RT-PCR. Result : 1. Morphologic changes: under the microscope, VSMC in control group was morphologic normal and well-distributed, the quantity of VSMC in experimental group decreased .2. Viability of VSMC : the viability of cells in experimental group was lower than that in control group(P0.05) 3. Rate of apotosis: the rate of apoptosis in experimental group was higher than that in control group(P0.05). 4. Expression of APP gene of VSMC : the expression of APP gene in experimental group was higher than that in control group (P0.01). Conclution : A β could damage SMC and upregulate the expression of APP gene. Key word : Amyloid β protein (A β) Vascular smooth muscle cells (VSMC) apoptosis Amyloid precursor protein (APP) gene 4 华 中 科 技 大 学 硕 士 学 位 论 文 引 言 脑淀粉样血管病（CAA ）是淀粉样物质沉积在脑内血管导致症状性脑血管功能 障碍的一种疾病，淀粉样物质主要沉积在脑皮质、软脑膜和小脑的小、中型动脉血管 中、外层的外侧部。病变血管可形成微动脉瘤、血管壁同心性裂开、慢性血管炎症和 纤维素样坏死。出血是该病最主要的临床表现，以脑叶出血多见，大脑枕、额、颞叶 最易受累。 Alonzo 等提出了CAA 形成的“种植”学说，指出A β种植在血管壁上及脑实质内， 随着A β在血管壁上沉积，血管内皮细胞和平滑肌细胞被破坏，开始由淀粉样物质取