三种麻醉剂对果蝇麻醉效果的比较.docVIP

晋 中 学 院 本科毕业论文（设计） 题 目 三种麻醉剂对果蝇麻醉 效果的比较 院 系 生物科学与技术学院 专 业 生物科学 姓 名 学 号 学习年限 指导教师 申请学位 理学 学士学位 2014年 4 月 10 日 三种麻醉剂对果蝇麻醉效果的比较 ——乙醚、乙醇、氯仿 学生姓名： 指导教师： 摘要 果蝇由于其饲养容易、生长迅速等诸多优点，是遗传学实验中常用的材料。在涉及果蝇的实验中，果蝇麻醉是很重要的环节。本实验旨在比较三种麻醉剂—乙醚、乙醇、氯仿对果蝇成虫、幼虫麻醉效果和复苏率以及乙醚、乙醇、氯仿对雌性果蝇和雄性果蝇的麻醉效果和复苏率率。从而选择果蝇麻醉实验中最适宜的麻醉剂，从而达到延长麻醉时间，并确保较高的苏醒率的目的。 关键词 果蝇 乙醚 乙醇 氯仿 麻醉时长 复苏率 Comparison of anesthetic effect of three kinds of anesthesia on Drosophila ——ether，alcohol，chloroformAuthor’s Name: Duan Ruifang Tutor:Li Hongyan ABSTRACT：Drosophila due to its easy feeding, rapid growth and many other advantages, is commonly used in genetics experimental course materials. In the fruit fly Drosophila to experiment, anesthesia is very important.The aim of this study is to compare three kinds of anesthetic ether, alcohol, chloroform, on Drosophila adults, larvae and the effect of anesthesia and resuscitation rate of ether, alcohol, chloroform in female Drosophila and male flies to the effect of anesthesia and recovery rate.In order to choose the most suitable anesthesia Drosophila anesthetic experiment, so as to extend the duration of anesthesia, and ensure the high recovery rate of. KEYWORDS：Drosophila ether alcohol chloroform the time of anesthesia recovery rate 目 录 1 实验材料及药品·······································1 1.1 实验材料 ··········································1 1.2 实验药品 ··········································1 2 试验方法··············································1 2.1 果蝇的培养与筛选··································1 2.2 果蝇的麻醉·········································2 2.3 重复实验···········································3 3 结果分析··············································3 注释·····················································3 参考文献······