遗传学（双语）徐吉臣-第10章.pdfVIP

Chapter 10 DNA replication and Synthesis 10.1 The mode of DNA replication 10.2 DNA Synthesis in vitro 10.3 DNA synthesis in Bacteria 10.4 Eukaryotic DNA synthesis 10.1 The mode of DNA replication Hypothesis: • Semiconservative replication Each replicated DNA molecule would consist of one old and one new strand • Conservative replication Two newly replicated strands are brought together, and the parental strands reassociate. • Dispersive replication The parental strands are dispersed into two new double helices following replication, and the resultant strand would the consist of both new and old DNA One round of replicationnew synthesis is shown in blue I. Experiment by J. Herbert Taylor, Philip Woods, Walter Hughes in 1957 Process: Material: root tips of Viciafaba (蚕豆) 3 Labeling during interphase: H-thymidinedTTP* Autoradiography replication I Disagree conservative replication replication II Disagree dispersive replication II. Experiment by Matthew Meselson and Franklin Stahl in 1958 Process: (1) Material: E.Coli, short life cycle (2) Medium and labeling: 15NH Cl medium, or 14NH Cl 4 4 medium DNA molecules with different Sedimentation (沉降) coefficient (3) Sedimentation equilibrium centrifugation density gradient centrifugation of cesium chloride （CsCl） 在离心力的作用下，梯度物质在离心管中形成了浓度和 密度梯度 （从上到下连续增高）, 不同样品颗粒按其各 自的比重平衡在相应的溶剂密度中形成区带。 15N/15N 14N/14N 15N/14N 14N/14N 15N/14N 15N/15N semiconservative replication 10.2 DNA Synthesis