棉花叶绿体基因组全序列微卫星分布规律研究-中国棉花杂志社.PDF

棉 花 学 报 Cotton Science 20 15袁27 6 冤院570―575 花叶绿体基因组全序列微卫星分布规律研究 蔡小彦 刘方 周忠丽 王星星 王春英 王玉红 王坤波* (棉花生物学国家重点实验室/ 中国农业科学院 花研究所 河南安阳455000) 摘要院利用已公布的陆地 渊 冤叶绿体基因组全序列测序结果 应用生物信息学软件对其叶 绿体微卫星的分布规律进行了全面分析遥 结果发现 陆地棉叶绿体基因组上共有72 个微卫星 主要分布于大 单拷贝区和小单拷贝区 且以单碱基微卫星为主 占总数的76.4% 曰二碱基微卫星占总数的20.8% 曰三碱基微卫 星占总数的2.8%遥 在单碱基微卫星中 又以A/T 重复为主 二者共占单碱基微卫星的92.7%遥 单碱基微卫星的 重复次数以10 12 次为主 二碱基微卫星的重复次数以5 6 次为主遥 本研究结果为开发 属植物叶绿体 用引物提供了理论依据遥 关键词院棉花曰叶绿体微卫星曰分布规律 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 20 15冤06-0570-06 10.11963/issn.1002-7807.20 1506009 Cai Xiaoyan, Liu Fang, Zhou Zhongli, Wang Xingxing, Wang Chunying, Wang Yuhong, Wang Kunbo* ( 455000 ) The distribution of chloroplast microsatellites in the chloroplast complete sequence of was ana- lyzed with bioinformatics software. The results showed that the chloroplast microsatellites of were mainly distribut- ed in the large single-copy region and the small single-copy region, and most microsatellites were complete repeats. Mononu- cleotide repeat motifs, dinucleotide repeat motifs and trinucleotide repeat motifs accounted for 76.4%, 20.8% and 2.8% of the to- tal, respectively. Repeats A and T were the main base in the mononucleotide motif, accounting for 92.7% of the total. The most common repeat number of (A)n and (T)n was 10 and 11, respectively, and the repeat number of the dinucleotide repeat motif was 5 or 6. These results are available for use in developing general primers from chloroplast DNA in . cotton; chloroplast microsatellites; distribution 微卫星DNA Microsatellite DNA 冤又称为简 速发展成为一种新型高效尧操作简单的分子标记 单重复序列 Simple sequence repeats, SSRs 冤袁是 技术遥 由于 具有微卫星标记的操作简单尧共显 指以少数几个核苷酸 一般为 1 6 个冤为基本重 性尧高多态性袁分布广泛性等优点又兼顾到叶绿 复单位的简单串联重复DNA 序列遥 它广泛分布 体基因组分子量小袁结构简单袁进化保守以及单 于真核